Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qianwen Gao
Date created: 2011-10-01 11:00:00
Date modified: 2015-05-08 01:13:03
3OC6HSL Sender and Receiver Device with lux pR-3/5 Driven by PLlac 0-1
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K658015_sequence (Version 1) |
Description
Quorum sensing is used by a number of gram-negative bacterial genera to regulate expression of specific sets of genes in a cell density-dependent fashion. We constructed the part which expresses LuxI and LuxR upon induction by isopropyl-b-D-thiogalactopyranoside (IPTG). There are two regulatory genes involved in quorum sensing, the I and R genes. The I gene directs the synthesis of an acyl-homoserine lactone (AHL) signal molecule termed the autoinducer. The R gene codes for a transcription factor that is responsive to the N-acyl HSL signal. Cells are permeable to this signal, and thus high cell densities are required to achieve a critical concentration of the autoinducer required to bind the luxR product, which will be combined with lux pR and then the expression downstream genes could be started. Site-directed mutagenesis has been used to modify the promoter lux pR in this signalling device. We have generated three point mutants by site-directed mutagenesis, designated lux pR-3 (BBa_K658006), lux pR-5(BBa_K658007) and lux pR-3/5(BBa_K658008). This signalling device is usually used to connected with some protein coding sequence,The promoter lux pR-5 (BBa_K658007) at the end of this sequence regulates the expression of downstream genes.Notes
This device is based on BBa_K658001. The RBS0.3 (BBa_B0032) in the killer protein producer regulate the expression of killer protein LacZalpha-ccdB. The higher strength a RBS has, the lower cell density may be got in the steady state.Source
F1610K658000