Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:07
galK::GFP generator ligated to TetR generator and constitutive sRNA device
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K737040_sequence (Version 1) |
Description
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one.[J23106,P0440]is a TetR generator, which represses tet promotor and can be induced by aTc.
aTc concentration gradient results in different sRNA Spot42 levels in cells when presented R0040, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42.
This device was mistaken for IPTG inducible sRNA device originally due to some ligation mistake,but we actually found this one is perfect to express full level sRNA to verify sRNA function without induction.
Notes
References:[1] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
Source
No| Access | Instance | Definition |
| public public public public public public public | BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | 1,35 44,883 892,945 955,1063 1072,1106 1115,1241 1250,1966 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |