Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2012-10-04 11:00:00
Date modified: 2015-05-08 01:13:15
pLac IPTG inducible generator
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K772104_sequence (Version 1) |
Description
This part is an inverting regulator sensitive to LacI and CAP.It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.
This part also produces LacI protein as an oscillator which results that as LacI protein is synthesized, the promoter wil be inhibited until the presence of IPTG. The inhibition will decrease the production of LacI which leads to the synthesis of this inhibitor.
>In the absence of LacI protein and CAP protein, this part promotes transcription.
>In the presence of LacI protein and CAP protein, this part inhibits transcription.
>LacI can be inhibited by IPTG.
>LacI is coded by BBa_C0010
Notes
Note that this part is not working unless IPTG is present in the media.Source
Registry| Access | Instance | Definition |
| public public | BBa_J04500 BBa_C0012 | BBa_J04500 lacI |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_C0012 | 1,220 227,1354 | BBa_J04500 lacI |