BBa_K784053

BBa_K784053 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K784053

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Asaf Blumental

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:22

theophylline riboswitch + T7*(T3) RNAP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K784053_sequence (Version 1)

Description

This part contains an engineered theophylline riboswitch (RS) (BBa K784005) and an engineered T7*(T3) RNA polymerase (K784052). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(K1F) RNAP only after inserting of theophylline.

Notes

The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(T3) RNAP are adjacent to one another without any restriction site between them.

Source

Assembly PCR of BBa K784005 and K784052

Components

Access	Instance	Definition
public	BBa_K784005	BBa_K784005
public	BBa_K784052	BBa_K784052

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K784005	1,74	BBa_K784005
BBa_K784052	75,2852	BBa_K784052

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K784053/1

Member of these Collections

• iGEM Parts Registry