sfGFP

BBa_K812331 Version 1

Component

This part has been discontinued.

Please consider using BBa_K812031 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K812331
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tristan Cerisy
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:28

sfGFP in pCS2+ with HSP70 promoter



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K812331_sequence (Version 1)

Description

The sfGFP protein is expressed under the inducible promoter Heat Shock Promoter 70 (HSP70). This part is complete and can be directly injected into fertilized frog's egg. To induce the expression the tadpole must be heat (for example from 21??C to 34??C).

Notes

pCS2+ is from K812000 and the promoter CMV was replaced by HSP70 with double digestion: SalI and HindIII digestion. HSP 70 is amplified with primers having the prefix SalI and the suffix HindIII.

Source

pCS2+ is from K812000. sfGFP is from Alphonso Jamarillo's lab, the Biobrick is K812031. HSP70 is from the article: Love, N.R. et al., 2011. pTransgenesis: a cross-species, modular transgenesis resource. Development (Cambridge, England), 138(24), pp.5451???8

Sequence Annotation Location Component / Role(s)
Kozak
sfGFP
stop
1,6
7,720
721,726
start_codon feature/start
feature/protein CDS
stop_codon feature/stop
igem#experience
Issues
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
isReplacedBy
BBa_K812031/1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K812331/1