Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:28
mycosporine-glycine biosynthetic pathway
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K814004_sequence (Version 1) |
Description
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG).This composite part contains protein generators for DHQS, O-MT and ATPG. E. coli transformed with this composite part are able to synthesize mycosporine-glycine, as detected by HPLC analysis of the media. 4-deoxygadusol is also detected in HPLC analysis.
Notes
Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota)for A. variabilis. From here, PCR primer extension was used to clone each individual gene with flanking restriction digest sites to be used for vector cloning. Each gene was cloned into an individual pUCBB BioBrick??? cloning vectors containing the lacP' promoter and an RBS. The resulting generators were then stacked into a single BioBrick??? cloning vector using classic assembly techniquesSource
All open reading frames in this composite part were taken from Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1).| Access | Instance | Definition |
| public public public | BBa_K814000 BBa_K814001 BBa_K814002 | BBa_K814000 BBa_K814001 BBa_K814002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K814000 BBa_K814001 BBa_K814002 | 1,1445 1454,3042 3051,4102 | BBa_K814000 BBa_K814001 BBa_K814002 |