Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Alvarado
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:33
Lysis device for R. opacus
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K836009_sequence (Version 1) |
Description
Lysis construct based on the one presented by Berkeley's 2008 team BBa_K112022 (http://partsregistry.org/wiki/index.php?title=Part:BBa_K112022. It is based on the inducible expression of a lysozyme and lambda phage's holin in order to liberate internal products of the bacterial cell in an efficient way. The induction is achieved by the addition of nitrile or a similar agent since both genes are regulated by nitA promoter.Notes
Constitutive expression of nitR and a low constitutive expression of an antiholin are required to avoid undesired lysis.Source
The majority of the parts where made by backtranslating a protein. Others are existing biobricks while some are promoters obtained from databases as NCBI. Check each individual part to know its origin.| Access | Instance | Definition |
| public public public public public public public public public public public | BBa_K836008 BBa_K836005 BBa_K836006 BBa_B1006 BBa_B1006 BBa_K143012 BBa_K836004 BBa_B1006 BBa_K143012 BBa_K836007 BBa_B1006 | nitA promo BBa_K836005 gpS BBa_B1006 BBa_B1006 Pveg gpS BBa_B1006 Pveg nitR BBa_B1006 |