pBAD-GFP

BBa_K845000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K845000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gr??gory Hansen
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:33

iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K845000_sequence (Version 1)

Description

In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.

The activity of the promoter was tested under several conditions (http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test)using a GFP as a reporter (on a pUA66).
We tested this activity in BW25113 ΔcyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).

Notes

Design note: http://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdf

Source

The part comes from the plasmid collection Alon (http://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdf).
However, pBAD is a natural promoter in E. Coli. It regulates the production of three proteins (AraA, AraB and AraD) which form the arabinose operon. Their production enables the use of arabinose as a carbon source.
It has six regulation sites, five of them are devoted to AraC fixation (three are repressing, one has a dual activity and one is an activator); the last one is a CRP binding site (positive activity).
This promoter is activated when both activated CRP and AraC are bind to it.


GFP comes from the protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence.
Aequorea victoria: is a jellyfish that can be found off the coast of north America.

Access Instance Definition
public
public
BBa_K845001
BBa_K845002
pBAD
GFP
Sequence Annotation Location Component / Role(s)
BBa_K845001
BBa_K845002
1,499
500,1242
pBAD
GFP
igem#experience None  
igem#sampleStatus It's complicated  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K845000/1