Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gr??gory Hansen
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:33
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K845000_sequence (Version 1) |
Description
In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.The activity of the promoter was tested under several conditions (http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test)using a GFP as a reporter (on a pUA66).
We tested this activity in BW25113 ΔcyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).
Notes
Design note: http://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdfSource
The part comes from the plasmid collection Alon (http://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdf).However, pBAD is a natural promoter in E. Coli. It regulates the production of three proteins (AraA, AraB and AraD) which form the arabinose operon. Their production enables the use of arabinose as a carbon source.
It has six regulation sites, five of them are devoted to AraC fixation (three are repressing, one has a dual activity and one is an activator); the last one is a CRP binding site (positive activity).
This promoter is activated when both activated CRP and AraC are bind to it.
GFP comes from the protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence.
Aequorea victoria: is a jellyfish that can be found off the coast of north America.
| Access | Instance | Definition |
| public public | BBa_K845001 BBa_K845002 | pBAD GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K845001 BBa_K845002 | 1,499 500,1242 | pBAD GFP |