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BBa_K855001

BBa_K855001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K855001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Akansha Mahavir Shah, Ma Tsz Shan

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:34

AHL-inducible Production of pvdQ

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K855001_sequence (Version 1)

Description

This part requires the external presence acyl homoserine lactones to stimulate the production of pvdQ (BBa_K855000).

Notes

The pvdQ gene contains an EcoRI-like site. This means that although the exact sequence of the EcoRI restriction enzyme is not present, EcoRI cuts the gene once. When digesting with EcoRI, a short incubation time is recommended to lessen the star activity.

Source

Pseudomonas aeruginosa PAO1

Components

Access	Instance	Definition
public public public public public public public public	BBa_R0011 BBa_B0034 BBa_C0062 BBa_B0015 BBa_R0062 BBa_B0034 BBa_K855004 BBa_B0015	lacI+pL BBa_B0034 luxr BBa_B0015 lux pR BBa_B0034 BBa_K855004 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011 BBa_B0034 BBa_C0062 BBa_B0015 BBa_R0062 BBa_B0034 BBa_K855004 BBa_B0015	1,54 64,75 82,837 871,999 1008,1062 1071,1082 1089,3395 3404,3532	lacI+pL BBa_B0034 luxr BBa_B0015 lux pR BBa_B0034 BBa_K855004 BBa_B0015

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K855001/1