Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: WU, CHENG-HSUAN
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:40
Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K887004_sequence (Version 1) |
Description
This part contain four enzymes. Each of the former three enzymes(alsS, ilvC& ilvD) linked with a zinc finger. The three enzymes could convert pyruvate into 2-ketoisoalerate(the Intermediate of isobutanol). The final enzyme(kivD)could change 2-ketoisoalerate into isobutyraldehyde, which would be converted into isobutanol by ADH in E.coli. This is a complete circuit to produce isobutanol.Notes
We add zinc fingers in front of enzymes in the circuit, and therefore the enzymes could bind the DNA program in order. It help enhance the efficiency of isobutanol production .Source
The circuit is optimized from 2011 NCTU_FORMOSA.| Access | Instance | Definition |
| public public public public public public public public public public public public public public public public public | BBa_R0010 BBa_B0034 BBa_K323105 BBa_K539713 BBa_B0034 BBa_K323107 BBa_K539711 BBa_B0034 BBa_K165006 BBa_K539712 BBa_K115002 BBa_C0040 BBa_J61048 BBa_R0040 BBa_B0032 BBa_K539714 BBa_B0015 | LacI BBa_B0034 BBa_K323105 BBa_K539713 BBa_B0034 BBa_K323107 BBa_K539711 BBa_B0034 BBa_K165006 BBa_K539712 BBa_K115002 tetR BBa_J61048 p(tetR) BBa_B0032 BBa_K539714 BBa_B0015 |