Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Deborah Huber
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:45
Constitutive expression of pABA
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K909014_sequence (Version 1) |
Description
PABA is derived from the aromatic branch point precursor chorismate in two steps. Aminodeoxychorismate (ADC) synthase converts chorismate and glutamine to ADC and glutamate. The conversion of chorismate and glutamine to ADC and glutamate determines the rate and therefore overexpression of ADC synthase leads to overproduction of pABA.ADC synthase is composed of two subunits, PabA and PabB. Finally ADC lyase removes pyruvate from ADC, aromatizes the ring, and generates PABA.
Notes
xyzSource
Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli. V K Viswanathan,; J M Green and; B P Nichols.| Access | Instance | Definition |
| public public public public public public public | BBa_J23100 BBa_B0034 BBa_K137006 BBa_B0034 BBa_K137005 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 BBa_K137006 BBa_B0034 pabA BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K137006 BBa_B0034 BBa_K137005 BBa_B0010 BBa_B0012 | 1,35 44,55 62,1951 1960,1971 1978,2562 2571,2650 2659,2699 | BBa_J23100 BBa_B0034 BBa_K137006 BBa_B0034 pabA BBa_B0010 BBa_B0012 |