Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alyssa Calder
Date created: 2010-04-19 11:00:00
Date modified: 2015-05-08 01:13:54
Zinc Promoter (ZntR regulated) with YFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M11524_sequence (Version 1) |
Description
Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound (1) with enhanced yellow fluorescent protein derived from A. victoria GFP as a reporter.Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
Notes
Composite part of BBa_K190016 and BBa_E0032Source
1) C.E. Outten, F.W. Outten, T.V. O???Halloran (1999) DNA Distortion Mechanism for Transcriptional Activation by ZntR, a Zn(II)-responsive MerR Homologue in Escherichia coli, The Journal of Biological Chemistry, Vol. 274, No. 53, Issue of December 31, pp. 37517???37524.| Access | Instance | Definition |
| public public public public public | BBa_K190016 BBa_B0034 BBa_E0032 BBa_B0010 BBa_B0012 | BBa_K190016 BBa_B0034 YFP BBa_B0010 BBa_B0012 |