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BBa_M1515

BBa_M1515 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_M1515

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brian Smith

Date created: 2012-04-18 11:00:00

Date modified: 2015-05-08 01:13:56

Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_M1515_sequence (Version 1)

Description

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL3. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Notes

See subparts. PstI site was removed

Source

See subparts.

Components

Access	Instance	Definition
public public public public public public public public public public public	BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1521 BBa_M1528 BBa_B0034 BBa_K208006 BBa_B0034 BBa_K208000 BBa_M1528 BBa_B0015	BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1521 BBa_M1528 BBa_B0034 BBa_K208006 BBa_B0034 BBa_K208000 BBa_M1528 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1521 BBa_M1528 BBa_B0034 BBa_K208006 BBa_B0034 BBa_K208000 BBa_M1528 BBa_B0015	1,220 229,414 423,434 441,839 848,4456 4465,4476 4485,4670 4679,4690 4697,5416 5425,9033 9042,9170	BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1521 BBa_M1528 BBa_B0034 BBa_K208006 BBa_B0034 BBa_K208000 BBa_M1528 BBa_B0015