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BBa_M30004

BBa_M30004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M30004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: natalie kuldell

Date created: 2005-11-24 12:00:00

Date modified: 2015-05-08 01:13:59

-- No description --

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Details

Types	DnaRegion
Roles	Generator engineered_region
Sequences	BBa_M30004_sequence (Version 1)

Description

lambda cI inverter (Q04510) directing transcription of mRFP (I13507). Constructed with standard assembly except PCR from VF and VR primers was used to generate Q04510 fragment with four-part inverter. Cells carrying M30004 are pink, however because PCR was used for cloning there may be silent mutations in the insert. M30004 is clone #2. A second clone will be saved as M30003.

Components

Access	Instance	Definition
public public public public public public public public public	BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012 BBa_R0051 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	BBa_B0034 cI lam BBa_B0010 BBa_B0012 cI lam BBa_B0034 mRFP1 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012 BBa_R0051 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	1,12 19,768 802,881 890,930 939,987 996,1007 1014,1694 1728,1807 1816,1856	BBa_B0034 cI lam BBa_B0010 BBa_B0012 cI lam BBa_B0034 mRFP1 BBa_B0010 BBa_B0012