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BBa_M30005

BBa_M30005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M30005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: natalie kuldell

Date created: 2005-12-04 12:00:00

Date modified: 2015-05-08 01:13:59

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Details

Types	DnaRegion
Roles	engineered_region Measurement
Sequences	BBa_M30005_sequence (Version 1)

Description

Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of P22 c2 gene. The P22 c2 protein represses expression of mRFP (M30001). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. A second isolate from this construction was saved as M30006.

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0082 BBa_B0034 BBa_C0053 BBa_B0010 BBa_B0012 BBa_R0053 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	OmpR BBa_B0034 c2 P22 BBa_B0010 BBa_B0012 cII p22 BBa_B0034 mRFP1 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0082 BBa_B0034 BBa_C0053 BBa_B0010 BBa_B0012 BBa_R0053 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	1,108 117,128 135,821 855,934 943,983 992,1045 1054,1065 1072,1752 1786,1865 1874,1914	OmpR BBa_B0034 c2 P22 BBa_B0010 BBa_B0012 cII p22 BBa_B0034 mRFP1 BBa_B0010 BBa_B0012