Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sophie Wong
Date created: 2007-02-27 12:00:00
Date modified: 2015-05-08 01:14:00
Refactored M13K07 from Hpa1 cut site to BamH1 cut site
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_M31518_sequence (Version 1) |
Description
Refactored part of the M13K07 genome from the Hpa1 cut site to the BamH1 cut site. It is designed to not contain overlaps of DNA elements. Also, each gene has a particular function assigned to it. It is also designed so that future manipulations can be made easier.Notes
Which restriction enzymes to include, which bases to mutate to eliminate overlaps of DNA elements.Source
M13K07 genome| Sequence Annotation | Location | Component / Role(s) |
| gene2 (ORF) promoter g5 promoter-g10 rbs-g10 gene10 (ORF) rbs-g5 g5 (ORF) BsrGI rbs-gVII gene VII promoter-g8 rbs-g9 gene IX SnaBI rbs-g8 g8 (ORF) ecoRI XmaI promoter-g3 rbs-g3_add gene III_add rbs-g3 gene III (ORF) BamHI promoter-g1 g1 rbs-g11 promoter-g4 PacI rbs g-4 g4 ApaLI MscI NgoMIV DrdI SgrAI SacII BssSI BstBI AhdI SacI HindIII HindIII BsmBI PvuI EcoNI NruI TliI XhoI Pst1 promoter-g2 SwaI rbs-g2 rbs-g1 AccI HpaI | 1,831 129,148 381,428 480,495 496,831 827,842 843,1106 1021,1025 1092,1107 1108,1209 1155,1201 1190,1205 1206,1304 1268,1273 1285,1300 1301,1522 1523,1528 1529,1534 1535,1582 1598,1613 1614,2255 2265,2280 2281,2922 2922,2922 3086,3132 3196,4242 3900,3915 4055,4103 4131,4138 4204,4219 4220,5500 4743,4748 5080,5085 5613,5617 5763,5765 5867,5871 6030,6035 6158,6162 6518,6520 6824,6834 6951,6956 7402,7406 7402,7407 7504,7508 7524,7526 7611,7612 7865,7870 7922,7926 7922,7926 8079,8083 8188,8235 8202,8209 8252,8267 8252,8267 8353,8358 1,1 | feature/protein CDS feature/promoter promoter feature/promoter promoter feature/rbs ribosome_entry_site feature/protein CDS feature/rbs ribosome_entry_site CDS feature/protein feature/misc sequence_feature feature/rbs ribosome_entry_site CDS feature/protein feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/protein feature/misc sequence_feature feature/rbs ribosome_entry_site feature/protein CDS feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter feature/rbs ribosome_entry_site feature/protein CDS feature/rbs ribosome_entry_site CDS feature/protein feature/misc sequence_feature feature/promoter promoter feature/protein CDS ribosome_entry_site feature/rbs promoter feature/promoter feature/misc sequence_feature feature/rbs ribosome_entry_site CDS feature/protein feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter feature/misc sequence_feature feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site sequence_feature feature/misc feature/misc sequence_feature |