Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandro Virrueta
Date created: 2010-06-05 11:00:00
Date modified: 2015-05-08 01:14:02
pTod + Purple Color Generator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M33355_sequence (Version 1) |
Description
Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. The catabolic genes of the toluene dioxygenase pathway are transcribed from a single promoter termed pTod, located upstream from the todX gene. The TodS/TodT TCS mediate pTod in response to toluene and other aromatic hydrocarbons. Once TodT is activated, it binds to a site on the pTod promoter approximately 107 base pairs upstream from the RNA polymerase-binding site. DNA bending caused by integration host factor (IHF) enables contact between TodT and RNA polymerase, initiating transcription.This part should be used in conjunction with BBa_M33350, the CDS for TodS downstream of an RBS, and BBa_M33354, the CDS for TodT downstream of an RBS.
Notes
NoneSource
The sequence of the Ptod promoter was given in the article referenced below. This identicle sequence was found upstream of the todX gene sequence natural existent in Pseudomonas putida given in http://www.ncbi.nlm.nih.gov/nuccore/AB042508.1 (todX coordinates: 14325..15686).| Access | Instance | Definition |
| public public | BBa_M33351 BBa_K274002 | BBa_M33351 BBa_K274002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M33351 BBa_K274002 | 1,116 125,7469 | BBa_M33351 BBa_K274002 |