BBa_M36257
BBa_M36257 Version 1 Component
This part has been discontinued.
Please consider using BBa_M36557 as a replacement.
Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elvis Ikwa, Alexis Garduno, Johnathan Bowes
Date created: 2012-12-04 12:00:00
Date modified: 2015-05-08 01:14:04
Vitamin D2 Actuator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36257_sequence (Version 1) |
Description
This part converts ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. In the presence of UV light ergosterol is converted to Vitamin D2. The coding region[BBa_M36655] produces C-24(28) sterol reductase an enzyme that catalyzes the final step in Vitamin D2 synthesis pathway by converting ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol.Notes
This construct uses a strong promoter and 5'UTR so that the amount of C-24(28) sterol reductase is substancial to efficiently convert the precursor to Vitamin D2.Source
This part was designed due to inspiration from [Tomoko Ikwaki,Haruyuki lefuji,Yoshikazu Hiraga,Akira Hosomi, Tomotake Morita, Yuko GigaHamam Kaoru Takegawa.???Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe???.7 December 2007 ] a direct link is available below http://mic.sgmjournals.org/content/154/3/830.full| Access | Instance | Definition |
| public public public | BBa_M36724 BBa_M36655 BBa_B0015 | BBa_M36724 BBa_M36655 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36724 BBa_M36655 BBa_B0015 | 1,85 92,1453 1462,1590 | BBa_M36724 BBa_M36655 BBa_B0015 |