BBa_M36257

BBa_M36257 Version 1

Component

This part has been discontinued.

Please consider using BBa_M36557 as a replacement.

Source: http://parts.igem.org/Part:BBa_M36257

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Elvis Ikwa, Alexis Garduno, Johnathan Bowes

Date created: 2012-12-04 12:00:00

Date modified: 2015-05-08 01:14:04

Vitamin D2 Actuator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_M36257_sequence (Version 1)

Description

This part converts ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. In the presence of UV light ergosterol is converted to Vitamin D2. The coding region[BBa_M36655] produces C-24(28) sterol reductase an enzyme that catalyzes the final step in Vitamin D2 synthesis pathway by converting ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol.

Notes

This construct uses a strong promoter and 5'UTR so that the amount of C-24(28) sterol reductase is substancial to efficiently convert the precursor to Vitamin D2.

Source

This part was designed due to inspiration from [Tomoko Ikwaki,Haruyuki lefuji,Yoshikazu Hiraga,Akira Hosomi, Tomotake Morita, Yuko GigaHamam Kaoru Takegawa.???Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe???.7 December 2007 ] a direct link is available below http://mic.sgmjournals.org/content/154/3/830.full

Components

Access	Instance	Definition
public	BBa_M36724	BBa_M36724
public	BBa_M36655	BBa_M36655
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_M36724	1,85	BBa_M36724
BBa_M36655	92,1453	BBa_M36655
BBa_B0015	1462,1590	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_M36557/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36257/1

Member of these Collections

• iGEM Parts Registry