Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Diana Gong, Michaela Hinks
Date created: 2014-10-23 11:00:00
Date modified: 2015-05-08 01:14:04
LasR-N-(3-oxododecanoyl)-homoserine lactone qsc102 sensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36268_sequence (Version 1) |
Description
Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasI produces N-(3-oxododecanoyl)-homoserine lactone, and LasR interacts with this molecule to transcriptionally regulate a variety of quorum sensing genes. One of these genes is qsc102. This composite part consists of a constitutive promoter, a RBS, the LasR gene, a terminator, and the qsc102 promoter. This entire construct is optimized for a plasmid that will be transformed into E. coli. This construct will be coupled to a standard fluorescence actuator so that in the presence of N-(3-oxododecanoyl)-homoserine lactone, the part will induce transcription of the fluorescence actuator.Notes
The device is optimized for E. coli, and a small sequence of "TAT" was entered between the RBS and the LasR gene for ideal transcription conditionsSource
See the sources documented in the individual parts of this device.| Access | Instance | Definition |
| public public public public public | BBa_M36266 BBa_M36269 BBa_M36264 BBa_M36135 BBa_M36265 | BBa_M36266 BBa_M36269 BBa_M36264 BBa_M36135 BBa_M36265 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36266 BBa_M36269 BBa_M36264 BBa_M36135 BBa_M36265 | 1,47 48,62 63,782 783,821 822,888 | BBa_M36266 BBa_M36269 BBa_M36264 BBa_M36135 BBa_M36265 |