Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ashley Henderson
Date created: 2014-10-23 11:00:00
Date modified: 2015-05-08 01:14:05
PoPS estrogen receptor beta and ERE operon.
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36558_sequence (Version 1) |
Description
This composite part is designed to code for the Estrogen Receptor Beta and has a promoter and operon at the end that is designed to arrest transcription of whatever gene follows the promoter at the end of our composite part when estrogen is detected. The parts that make up the composite are the promoter, which is a strong, general E. coli promoter, a medium strength ribosome binding site, the cDNA for estrogen receptor beta, the stop codon, the general terminator which arrests transcription, the strong, general E. coli promoter for the gene that follows ER beta, and the ERE operon that ER beta can bind to to arrest transcription of the gene that follows the ER beta gene.Notes
In our design we ordered the basic parts into a precise order that would allow the ER beta to be made and able to arrest the transcription of the the gene that follows by binding to the ERE operon.Source
The parts that make up this composite are as follows and in this order:BBa_J23110 BBa_M36559 BBa_M36560 BBa_M36561 BBa_J23104 BBa_M36562
They come from both the parts registry and are edited basic parts that we manually added to the registry.
| Access | Instance | Definition |
| public public public public public public | BBa_J23110 BBa_M36559 BBa_M36560 BBa_M36561 BBa_J23104 BBa_M36562 | BBa_J23110 BBa_M36559 BBa_M36560 BBa_M36561 BBa_J23104 BBa_M36562 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_M36559 BBa_M36560 BBa_M36561 BBa_J23104 BBa_M36562 | 1,35 36,54 55,1647 1648,1727 1728,1762 1763,1777 | BBa_J23110 BBa_M36559 BBa_M36560 BBa_M36561 BBa_J23104 BBa_M36562 |