Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dallin V. Christensen
Date created: 2015-04-22 11:00:00
Date modified: 2015-05-08 01:14:09
pRM promoter with phiR73 delta that is used to activate the promoter PF
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M45505_sequence (Version 1) |
Description
Lambda Prm promoter was modified to be activated but not repressed by the lambda repressor Cl. This gives the promoter the ability to be sense when a phage has infected the cell. The delta activator, designed by Cambridge 2007, was taken from phiR73 phage and acts on a class of inducible promoters (parts I746360 to I746365). The part sequence does already contain a ribosome binding site (B0034). A double stop codon B0015 is also added to the back of the part.Notes
noneSource
phiR73 phage, Lambda phage| Access | Instance | Definition |
| public public public | BBa_I12007 BBa_I746352 BBa_B0015 | Prm + BBa_I746352 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I12007 BBa_I746352 BBa_B0015 | 1,82 91,354 363,491 | Prm + BBa_I746352 BBa_B0015 |