BBa_K1859008
BBa_K1859008 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*2 linker

BBa_K1859013
BBa_K1859013 Version 1 (Component)
3*GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

IodoY RS
BBa_K1416001 Version 1 (Component)
The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon

BBa_K1363601
BBa_K1363601 Version 1 (Component)
the gate of yes of RNA logic gates

BBa_M31541
BBa_M31541 Version 1 (Component)
This is the M13K07 genome refactored between the Hap1 and Bam1 Sites

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K309009
BBa_K309009 Version 1 (Component)
pOdr-10 (for use in C. elegans chassis)

BBa_K309014
BBa_K309014 Version 1 (Component)
pOdr-1 (for use in C. elegans chassis)

BBa_K309017
BBa_K309017 Version 1 (Component)
pStr-220 (for use in C. elegans chassis)

BBa_K309011
BBa_K309011 Version 1 (Component)
pHsp-3 (for use in C. elegans chassis)

BBa_K1114000
BBa_K1114000 Version 1 (Component)
The MoClo format of BBa_J23100 with AB fusion sites.

BBa_K669007
BBa_K669007 Version 1 (Component)
Allows the measurement of the ChiP promoter activity

BBa_K669006
BBa_K669006 Version 1 (Component)
Allows the measurement of the CBP promoter activity

BBa_K1631003
BBa_K1631003 Version 1 (Component)
Tlanslational unit of Colicin Lysis Protein (for colicin-E3)

BBa_I716057
BBa_I716057 Version 1 (Component)
-CmR-RBS library-beta HbA-

BBa_I716161
BBa_I716161 Version 1 (Component)
[Library RBS]hemA from R.cap

BBa_I716056
BBa_I716056 Version 1 (Component)
-kan-RBS library-alpha HbA-

BBa_I716160
BBa_I716160 Version 1 (Component)
[Library RBS]hemA from CFT073

R37
BBa_K780002 Version 1 (Component)
Strong RBS for Bacillus Subtilis

BBa_K531006
BBa_K531006 Version 1 (Component)
Optimized esp gene for <i>C. crescentus</i>

BBa_K189001
BBa_K189001 Version 1 (Component)
Nu1 and A of lambda genome

BBa_K1465213
BBa_K1465213 Version 1 (Component)
RubisCO of Halothiobacillus neapolitanus under control of the ptac promoter

BBa_K957004
BBa_K957004 Version 1 (Component)
nah Operon for degradation of naphthalene to salicylate

BBa_K1613006
BBa_K1613006 Version 1 (Component)
A composite part that can detect some toxins.

BBa_K1613007
BBa_K1613007 Version 1 (Component)
A composite part that can detect some toxins.

BBa_K1613005
BBa_K1613005 Version 1 (Component)
A composite part that can detect some toxins.

BBa_K103013
BBa_K103013 Version 1 (Component)
linker fused to omega fragment of TEM beta-lactamase

BBa_K103009
BBa_K103009 Version 1 (Component)
linker fused to alpha fragment of TEM beta-lactamase

BBa_K1707028
BBa_K1707028 Version 1 (Component)
CFP-ssRA downstream of a cI regulated promoter

BBa_K736001
BBa_K736001 Version 1 (Component)
TAT signal sequence fused to red fluorescent protein (E1010)

BBa_K1361003
BBa_K1361003 Version 1 (Component)
Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C

BBa_K1172908
BBa_K1172908 Version 1 (Component)
Part 1 of the Biosafety-System Lac of Growth (pRha lacI alr)

BBa_K631006
BBa_K631006 Version 1 (Component)
NPY1R_Rat (GPCR, for use in C. elegans)

BBa_K631008
BBa_K631008 Version 1 (Component)
MAM1R (GPCR, for use in C. elegans)

BBa_K631005
BBa_K631005 Version 1 (Component)
NPY1R_Mus (GPCR, for use in C. elegans)

BBa_J61140
BBa_J61140 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_J61145
BBa_J61145 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_J61144
BBa_J61144 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_J61143
BBa_J61143 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_J61142
BBa_J61142 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_J61147
BBa_J61147 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_J61141
BBa_J61141 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_J61146
BBa_J61146 Version 1 (Component)
{rbs1} Library Member in BBb

BBa_K1778006
BBa_K1778006 Version 1 (Component)
Kana-His is a selective marker gene in experiment.

BBa_I1060
BBa_I1060 Version 1 (Component)
Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition)

BBa_K2057003
BBa_K2057003 Version 1 (Component)
LasR under the regulation of the promoter chiA74

BBa_K1407026
BBa_K1407026 Version 1 (Component)
detect the intensity of precA

BBa_K740003
BBa_K740003 Version 1 (Component)
promoter with loxP sites that can switch the reporter

BBa_I742134
BBa_I742134 Version 1 (Component)
Recombinase XerC of the XerCD complex.

BBa_K233318
BBa_K233318 Version 1 (Component)
A tester for Snowdrift 1.