BBa_K568003
BBa_K568003 Version 1 (Component)
T7 promoter lacZ reporter part

BBa_K568006
BBa_K568006 Version 1 (Component)
Intermediate synthetic part of optogenetical AND-Gate

aspA
BBa_C0083 Version 1 (Component)
aspartate ammonia-lyase

BBa_K581000
BBa_K581000 Version 1 (Component)
sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)

ChiPm-lacZ
BBa_K564013 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K564016
BBa_K564016 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K564017
BBa_K564017 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

mdnA-myc-g
BBa_K627006 Version 1 (Component)
Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

AraC_14_3C
BBa_K627011 Version 1 (Component)
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease

BBa_K654030
BBa_K654030 Version 1 (Component)
Anderson 1.0 Promoter - Promoter only part

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_K581005
BBa_K581005 Version 1 (Component)
Pc+sgrS(wt)+Terminator (small RNA regulator, conjugate part of ptsG(wt))

BBa_S01291
BBa_S01291 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01293
BBa_S01293 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01294
BBa_S01294 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01295
BBa_S01295 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01296
BBa_S01296 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01297
BBa_S01297 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01298
BBa_S01298 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01299
BBa_S01299 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01300
BBa_S01300 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01301
BBa_S01301 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01302
BBa_S01302 Version 1 (Component)
Intermediate part from assembly 236

BBa_S01303
BBa_S01303 Version 1 (Component)
Intermediate part from assembly 236

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

BBa_K758004
BBa_K758004 Version 1 (Component)
GAL4, this part coding protein binds and activates the UAS sequence

BBa_S01304
BBa_S01304 Version 1 (Component)
Intermediate part from assembly 236

UAS-LacZ
BBa_K758005 Version 1 (Component)
UAS LacZ, this part expresses LacZ in the presence of GAL4

UAS-EGFP
BBa_K758006 Version 1 (Component)
UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.

BBa_S03419
BBa_S03419 Version 1 (Component)
--Specify Parts List--

BBa_K737003
BBa_K737003 Version 1 (Component)
This part is polyphosphate kinase.

BBa_K737016
BBa_K737016 Version 1 (Component)
This part conteins gvpA protein's coding sequence.

BBa_K737017
BBa_K737017 Version 1 (Component)
This part conteins gvpC-20psi protein

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K739004
BBa_K739004 Version 1 (Component)
Ax21 protein - signaling part

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

BBa_K774002
BBa_K774002 Version 1 (Component)
Comparator Circuit Part 1

BBa_K774003
BBa_K774003 Version 1 (Component)
Comparator Circuit Part 2

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Cello E. Coli Parts
Eco1C1G1T1_collection Version 1 (Collection)
These are the Cello parts for E. Coli circuits

BBa_S04142
BBa_S04142 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022)

BBa_S04143
BBa_S04143 Version 1 (Component)
The Partial Butanol Operon (Parts I725023+I725024)

BBa_S04144
BBa_S04144 Version 1 (Component)
The Partial Butanol Operon (Parts I725024+I725025)

BBa_S04145
BBa_S04145 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022+I725023)

BBa_K106671
BBa_K106671 Version 1 (Component)
GFP, Aar1 AD part