BBa_I756010
BBa_I756010 Version 1 (Component)
SV40 Introng with LacO Sites

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_K136056
BBa_K136056 Version 1 (Component)
RBS - lasI - ECFP Tripart (+LVA)

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

FBS-AceB+L
BBa_K1163999 Version 1 (Component)
Inverter composed of Fur Binding site from AceB promoter + LacI-LVA

BBa_K658021
BBa_K658021 Version 1 (Component)
GFP driven by lacl+pL

BBa_K794001
BBa_K794001 Version 1 (Component)
CSP(derived from E. coli) - lasI

BBa_K1767003
BBa_K1767003 Version 1 (Component)
P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K1675023
BBa_K1675023 Version 1 (Component)
P-atp2 mutant 706-B0034-lacz alpha

BBa_K1675022
BBa_K1675022 Version 1 (Component)
P-atp2 mutant 317-B0034-lacz alpha

BBa_K1675025
BBa_K1675025 Version 1 (Component)
P-atp2 mutant 399-B0034-lacz alpha

BBa_K1675024
BBa_K1675024 Version 1 (Component)
P-atp2 mutant 389-B0034-lacz alpha

BBa_K1675020
BBa_K1675020 Version 1 (Component)
P-atp2 mutant 226-B0034-lacz alpha

BBa_K086000
BBa_K086000 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter - YFP

BBa_K329005
BBa_K329005 Version 1 (Component)
Strong RBS (B0034) - LacZ-alpha fragment (I732006)

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_I732091
BBa_I732091 Version 1 (Component)
Double Repoters (LacZ-alpha and GFP-AAV)

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K564016
BBa_K564016 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K564017
BBa_K564017 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_I715071
BBa_I715071 Version 1 (Component)
Lac promoter with Ribosomal Binding Site

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

BBa_K1952012
BBa_K1952012 Version 1 (Component)
Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

BBa_K584011
BBa_K584011 Version 1 (Component)
Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP

BBa_J40000
BBa_J40000 Version 1 (Component)
Quorum sensing promoter with lac I and CFP

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1222004
BBa_K1222004 Version 1 (Component)
pCam(T7 promoter+lac operator+CamR antisense 2+T7 terminator)

BBa_K2007001
BBa_K2007001 Version 1 (Component)
pLR promoter expressing GFP and Lac promoter expressing FadR gene

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.