BBa_K415005BBa_K415005 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term
BBa_K223033BBa_K223033 Version 1 (Component)trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer
BBa_J119408BBa_J119408 Version 1 (Component)Pupp promoter mutant - Substitution of C and G to A at 28 and 30
BBa_K202004BBa_K202004 Version 1 (Component)Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 3
BBa_K199194BBa_K199194 Version 1 (Component)TT-pLux-RBS-LuxI 2 sites-RBS-GFP-RBS-RFP-pLacI-RBS-LuxR
BBa_K1022125BBa_K1022125 Version 1 (Component)pT7: RBS: His6- SUMO: Signiferin: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis
BBa_K199193BBa_K199193 Version 1 (Component)TT-pLux-RBS-LuxI 2 sites-RBS-RFP-RBS-GFP-pBAD-RBS-LuxR
BBa_K1088059BBa_K1088059 Version 1 (Component)GFP reporter with flexible linker at N-terminus for creation of GFP fusions
BBa_K1088052BBa_K1088052 Version 1 (Component)GFP reporter with flexible linker at N-terminus for creation of GFP fusions
BBa_K1178000BBa_K1178000 Version 1 (Component)tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon
BBa_J100017BBa_J100017 Version 1 (Component)TT+pLux+RBS+LuxI(2-SAT 2 clause)+RBS+GFP+pLac+RBS+LuxR+tRNAs
BBa_K1022126BBa_K1022126 Version 1 (Component)pT7: RBS: His6- SUMO: Magainin II: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis
IodoY RSBBa_K1416001 Version 1 (Component)The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon
BBa_K1361005BBa_K1361005 Version 1 (Component)CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive
BBa_J107021BBa_J107021 Version 1 (Component)aTc sensor (J23106 promoter) with GFP
BBa_K368020BBa_K368020 Version 1 (Component)attB (bacterial attachment site, recombined by PhiC31 Integrase)
BBa_J31016BBa_J31016 Version 1 (Component)part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t
BBa_K199112BBa_K199112 Version 1 (Component)RFP-TT intermediate
BBa_K660605BBa_K660605 Version 1 (Component)OmpF + wYFP + TT
BBa_M36556BBa_M36556 Version 1 (Component)5' Bicistronic UTR (medium), does not include ATG start
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K318501BBa_K318501 Version 1 (Component)lacI pL + RBS + RcsB + TT
BBa_K199165BBa_K199165 Version 1 (Component)pT7 + RBS + GFP + TT in pSB3T5
BBa_J107055BBa_J107055 Version 1 (Component)pSB3K3 with RBS-RFP-TT between SpeI-PstI
BBa_S03737BBa_S03737 Version 1 (Component)pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.