BBa_J72186
BBa_J72186 Version 1 (Component)
TrimR, pir expression cassette for genomic integration

BBa_J72188
BBa_J72188 Version 1 (Component)
weak violacein expression cassette

BBa_J72189
BBa_J72189 Version 1 (Component)
strong violacein expression cassette

BBa_J72190
BBa_J72190 Version 1 (Component)
neuS expression cassette

BBa_J72191
BBa_J72191 Version 1 (Component)
wbbL expression cassette

BBa_J72192
BBa_J72192 Version 1 (Component)
manB and neuS expression cassette

BBa_J72193
BBa_J72193 Version 1 (Component)
manB expression cassette

BBa_J72194
BBa_J72194 Version 1 (Component)
neuS expression cassette

BBa_J72195
BBa_J72195 Version 1 (Component)
Barnase/Barstar expression cassette

BBa_J72199
BBa_J72199 Version 1 (Component)
wbbL neuS expression cassette

BBa_J72200
BBa_J72200 Version 1 (Component)
hlyE expresion cassette

BBa_J72201
BBa_J72201 Version 1 (Component)
BamHI endonuclease expression cassette

BBa_K758003
BBa_K758003 Version 1 (Component)
UAS, this part consists of five UAS sequences and hsp70 TATA.

BBa_K817018
BBa_K817018 Version 1 (Component)
gamma-delta resolvase cassette

BBa_K817015
BBa_K817015 Version 1 (Component)
srnBC toxin-antitoxin cassette

BBa_K812013
BBa_K812013 Version 1 (Component)
GFP-AID OsTirI polysistronic system for auxin detection in tadpole

CBDcex(T7)
BBa_K863102 Version 1 (Component)
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)

gpS
BBa_K836004 Version 1 (Component)
Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K124017
BBa_K124017 Version 1 (Component)
Bacteriophage Lysis Cassette S105, R, and Rz

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K142029
BBa_K142029 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette

BBa_K142030
BBa_K142030 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette

BBa_K142031
BBa_K142031 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette

BBa_K142073
BBa_K142073 Version 1 (Component)
lac-controlled GFP generator with constitutive lacI expression cassette

BBa_K142074
BBa_K142074 Version 1 (Component)
lac-controlled GFP-LVA generator with constitutive lacI expression cassette

BBa_K142075
BBa_K142075 Version 1 (Component)
lac-controlled CFP generator with constitutive lacI expression cassette

BBa_K142076
BBa_K142076 Version 1 (Component)
lac-controlled RFP generator with constitutive lacI expression cassette

BBa_K142048
BBa_K142048 Version 1 (Component)
promotorless lacI expression cassette followed by constitutively active TetR generator

BBa_K112021
BBa_K112021 Version 1 (Component)
Lambda phage lysis device - no promoter

BBa_K112022
BBa_K112022 Version 1 (Component)
Lambda phage lysis device - no promoter

BBa_K112023
BBa_K112023 Version 1 (Component)
Lambda phage lysis device - no promoter

BBa_K112802
BBa_K112802 Version 1 (Component)
T4 phage lysis device

BBa_K112130
BBa_K112130 Version 1 (Component)
Xis.Int with RBS, TrfA with RBS and terminator

BBa_K122021
BBa_K122021 Version 1 (Component)
Cassette3: Constitutive TAL with integration site

trfA
BBa_K112131 Version 1 (Component)
TrfA constitutive expression cassette

BBa_K112133
BBa_K112133 Version 1 (Component)
Xis Int temperature sensitive expression cassette

BBa_K112137
BBa_K112137 Version 1 (Component)
Xis Int & TrfA combined expression cassette

BBa_J70028
BBa_J70028 Version 1 (Component)
tetM tetracycline resistance cassette

BBa_K112808
BBa_K112808 Version 1 (Component)
Enterobacteria phage T4 Lysis Device - no promoter

BBa_K112147
BBa_K112147 Version 1 (Component)
lambda lysis device controlled by magnesium promoter

BBa_K112146
BBa_K112146 Version 1 (Component)
lambda lysis device controlled by PmgtCB magnesium promoter