BBa_K833014
BBa_K833014 Version 1 (Component)
constitutive promoter followed by a lox site with a bidirectional terminator and then a lox site.

BBa_K2176001
BBa_K2176001 Version 1 (Component)
Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A

BBa_K079049
BBa_K079049 Version 1 (Component)
GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator

BBa_K531010
BBa_K531010 Version 1 (Component)
Constitutive promoter and RBS BBa_K081005 + <i>Caulobacter</i> optimized <i>dspB</i> + <i>rsaA</i> C

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

pTEF
BBa_K124001 Version 1 (Component)
Yeast TEF2 Constitutive Promoter

BBa_K934000
BBa_K934000 Version 1 (Component)
Constitutive LuxR generator

BBa_K2052016
BBa_K2052016 Version 1 (Component)
FimH site directed mutated with RPMrel and ButCoat

BBa_K888002
BBa_K888002 Version 1 (Component)
Constitutive promoter Assembly Standard 21.2

BBa_K395102
BBa_K395102 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)

BBa_K395103
BBa_K395103 Version 1 (Component)
GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)

BBa_K311000
BBa_K311000 Version 1 (Component)
Constitutive tet promoter with mRFP

BBa_K660612
BBa_K660612 Version 1 (Component)
Strong Constitutive Promoter + Strong RBS

BBa_K206015
BBa_K206015 Version 1 (Component)
constitutive promoter-RBS (J23100:B0030)

BBa_K272001
BBa_K272001 Version 1 (Component)
Constitutive Expression Cassette for RFP

BBa_K584025
BBa_K584025 Version 1 (Component)
Constitutive Promotor (J23119) + GFP generator

BBa_K1088001
BBa_K1088001 Version 1 (Component)
Dxs reporter system with constitutive promoter

BBa_K1719014
BBa_K1719014 Version 1 (Component)
Constitutive promoter family member and RBS

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

BBa_K1650000
BBa_K1650000 Version 1 (Component)
Constitutive promoter expressing GFP (ILS 2015)

BBa_I766223
BBa_I766223 Version 1 (Component)
HMTM1-EE under weak constitutive promoter

BBa_K311001
BBa_K311001 Version 1 (Component)
Strong and constitutive tet promoter with downstream mRFP

placIQ RBS
BBa_K193604 Version 1 (Component)
GFP behind a constitutive promoter (placIQ) on pSB4A5

BBa_I719021
BBa_I719021 Version 1 (Component)
Constitutive 3OC₆HSL Sender Device

BBa_J47053
BBa_J47053 Version 1 (Component)
Constitutive device (medium transcription) for lacI repressor, strong RBS

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_K332034
BBa_K332034 Version 1 (Component)
Constitutive promoter + 37&#8451; induced RBS + tetR + double terminator

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K079032
BBa_K079032 Version 1 (Component)
GFP reporter protein under the control of the BBa_J23100 constitutive promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.