BBa_K648131
BBa_K648131 Version 1 (Component)
Pr of Or tester with cI repressor

BBa_K338062
BBa_K338062 Version 1 (Component)
GFP Reporter Device, Heat-Shock or IPTG Induced

BBa_K327017
BBa_K327017 Version 1 (Component)
lux OR delta cI activated "off" part

BBa_K541000
BBa_K541000 Version 1 (Component)
Limulus anti-LPS factor (LALF) for Bacillus subtilis

BBa_K077025
BBa_K077025 Version 1 (Component)
HSL/LuxR or LasR promotor test system

BBa_J70368
BBa_J70368 Version 1 (Component)
I13502 or K093005 (B0034+E1010) rfp without PsrI sites

Holin, T4
BBa_K112003 Version 1 (Component)
T4 holin, without start or stop codon, berkeley standard

BBa_K415004
BBa_K415004 Version 1 (Component)
P_const(high) : RBS : LuxR : Term : pLux/CI-OR

BBa_K1132038
BBa_K1132038 Version 1 (Component)
XOR-inverted RFP gate (BBa_K1132035) with T7 polymerase under the control of a strong promoter

BBa_M31748
BBa_M31748 Version 1 (Component)
Gene VII w/o Genes IX RBS or VIII Promoter

BBa_J70552
BBa_J70552 Version 1 (Component)
J70551.J70549 (or J70548.J70550.J70549) composite part

BBa_K750108
BBa_K750108 Version 1 (Component)
PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose

BBa_K2123301
BBa_K2123301 Version 1 (Component)
Novel Synthetic Phytochelatin codon optimized for E. coli expression

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

BBa_M31002
BBa_M31002 Version 1 (Component)
Refactored Gene 2 without Gene 10's Promoter or RBS

BBa_K1363611
BBa_K1363611 Version 1 (Component)
key-1 of gate-of-or of RNA logic gates

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K112222
BBa_K112222 Version 1 (Component)
{< ihfB >} The ihf beta gene without start or stop codons, BBb format

BBa_K415003
BBa_K415003 Version 1 (Component)
P_const(low/med) : RBS : LuxR : Term : pLux/CI-OR

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_J70556
BBa_J70556 Version 1 (Component)
BsaXI test circuit: J70555.J70554 or J70554.J70550.J70554

BBa_J70084
BBa_J70084 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70030 (PpiI) in pSB1AT3

BBa_K648125
BBa_K648125 Version 1 (Component)
Or tester with cI repressor with slightly weak RBS

BBa_K648126
BBa_K648126 Version 1 (Component)
Or tester with cI repressor with very weak RBS

BBa_K1633002
BBa_K1633002 Version 1 (Component)
MOR siRNA 3

BBa_K1633000
BBa_K1633000 Version 1 (Component)
MOR siRNA 1

BBa_K1633001
BBa_K1633001 Version 1 (Component)
MOR siRNA 2

BBa_M11402
BBa_M11402 Version 1 (Component)
5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K251000
BBa_K251000 Version 1 (Component)
coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)

BBa_I714001
BBa_I714001 Version 1 (Component)
trwC of R388 ( relaxase or nickase of the R388 conjugative plasmid )

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

BBa_K415002
BBa_K415002 Version 1 (Component)
P_const(high) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term

xis domain
BBa_K112203 Version 1 (Component)
{< xis >} The bacteriophage lambda xis gene without start or stop codons; assembly standard 21

BBa_J70559
BBa_J70559 Version 1 (Component)
N-terminal fusion test: R0010:J70558 or R0010:J70550:E0040

BBa_K363002
BBa_K363002 Version 1 (Component)
A calcium dependent response element binding site for the Crz1 activator

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K2123114
BBa_K2123114 Version 1 (Component)
Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K1437006
BBa_K1437006 Version 1 (Component)
mouse AhR detective protein 83-805 with terminator codon optimized for yeast

BBa_K802003
BBa_K802003 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

BBa_K2016006
BBa_K2016006 Version 1 (Component)
Superfolder GFP under control or medium constitutive promoter and RyhB siRNA domain

BBa_K415001
BBa_K415001 Version 1 (Component)
P_const(low/med) : LuxR : Term : pLux/CI-OR : RBS-mCherry : Term

BBa_K750115
BBa_K750115 Version 1 (Component)
pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K648130
BBa_K648130 Version 1 (Component)
Pr of Or tester with cI repressor with very weak RBS