BBa_K1695049BBa_K1695049 Version 1 (Component)pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz
CsgDBBa_K1019001 Version 1 (Component)CsgD: positive regulator of curlin genes
BBa_K1923007BBa_K1923007 Version 1 (Component)Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene
BBa_K546547BBa_K546547 Version 1 (Component)Constitutive (tetR repressible) LacI and RFP expression
BBa_I13972BBa_I13972 Version 1 (Component)Lux CFP Protein Generator (R0062.E0420)
BBa_I13971BBa_I13971 Version 1 (Component)c1 CFP Protein Generator (R0051.E0420)
BBa_I758601BBa_I758601 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_J107040BBa_J107040 Version 1 (Component)PtetR expressing GFP
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_K1438010BBa_K1438010 Version 1 (Component)Bacterial Iron Storage Expressor
BBa_K1707026BBa_K1707026 Version 1 (Component)CFP-ssRA under the control of J23101
BBa_K2144011BBa_K2144011 Version 1 (Component)Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter
BBa_K812133BBa_K812133 Version 1 (Component)sfGFP with kozak sequence for expression in Xenopus
BBa_K2007001BBa_K2007001 Version 1 (Component)pLR promoter expressing GFP and Lac promoter expressing FadR gene
BBa_K2123117BBa_K2123117 Version 1 (Component)Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato
BBa_K415005BBa_K415005 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term
BBa_K581003BBa_K581003 Version 1 (Component)SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
BBa_K2123301BBa_K2123301 Version 1 (Component)Novel Synthetic Phytochelatin codon optimized for E. coli expression
BBa_J40000BBa_J40000 Version 1 (Component)Quorum sensing promoter with lac I and CFP
BBa_K549004BBa_K549004 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_I741016BBa_I741016 Version 1 (Component)Reverse Total XylR Transcriptional Regulator Left Facing (I741011 reverse complement
BBa_K812130BBa_K812130 Version 1 (Component)Citrine reporter with a Kozak sequence for expression in Xenopus
BBa_K563053BBa_K563053 Version 1 (Component)vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
pSBBs0KBBa_K823026 Version 1 (Component)pSB<sub>Bs</sub>0K-P<sub>spac</sub> (replicative Bacillus subtilis expression vector; IPTG inducible
BBa_K1412088BBa_K1412088 Version 1 (Component)A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit
BBa_K1650000BBa_K1650000 Version 1 (Component)Constitutive promoter expressing GFP (ILS 2015)
BBa_J329001BBa_J329001 Version 1 (Component)Simple lux-based QS circuit expressing untagged GFP
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.