BBa_I1070
BBa_I1070 Version 1 (Component)
XOR test/debug module

BBa_K2057004
BBa_K2057004 Version 1 (Component)
LasR under the regulation of the promoter BtI-BtII

BBa_K525582
BBa_K525582 Version 1 (Component)
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic

BBa_K260018
BBa_K260018 Version 1 (Component)
Cell free Chassis: Mass production of aqueous vesicles in oil phase

BBa_G0007
BBa_G0007 Version 1 (Component)
Short sequence to promoter addition of 3' A overhang during PCR

BBa_G0008
BBa_G0008 Version 1 (Component)
Short sequence to promoter addition of 3' A overhang during PCR

BBa_K1326006
BBa_K1326006 Version 1 (Component)
Plac + POR + DVR1

BBa_K2151003
BBa_K2151003 Version 1 (Component)
pHLBA-E5501: GFP under control of strong constitutive promoter

BBa_K215210
BBa_K215210 Version 1 (Component)
IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin

BBa_K2151004
BBa_K2151004 Version 1 (Component)
p25-E5501: GFP under control of medium constitutive promoter

BBa_K2151005
BBa_K2151005 Version 1 (Component)
p32-E5501: GFP under control of strong constitutive promoter

BBa_I20243
BBa_I20243 Version 1 (Component)
Autoregulated synthesis of T7 RNAP with lacI negative feedback

BBa_K195406
BBa_K195406 Version 1 (Component)
This part is composed of F10scFv and C-IgAP

Scaffold g
BBa_K1602029 Version 1 (Component)
Inducible generator of protein scaffold with N-terminal HisTag

BBa_K831007
BBa_K831007 Version 1 (Component)
Inducible MqsR toxin under the control of tetR promoter

BBa_K831006
BBa_K831006 Version 1 (Component)
Inducible MqsR toxin under the control of tetR promoter

BBa_K1465105
BBa_K1465105 Version 1 (Component)
Fumarate reductase (Fum) under control of the T7 promoter

BBa_K2082117
BBa_K2082117 Version 1 (Component)
Six mutator genes under control of a modified Pbad

BBa_K831015
BBa_K831015 Version 1 (Component)
Inducible HipA7 toxin under the control of prm promoter

BBa_K215211
BBa_K215211 Version 1 (Component)
IPTG Inducible 5tmr OmpA Surface Display of WT Streptavidin

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Switch
BBa_K1185000 Version 1 (Component)
B.subtilis Rod to L-form Switch

BBa_K091213
BBa_K091213 Version 1 (Component)
LacI X86 and pLac promoter upstream of GFP

BBa_K091210
BBa_K091210 Version 1 (Component)
lacIQ1 promoter under the control of LacI repressor

BBa_K091211
BBa_K091211 Version 1 (Component)
lacIQ promoter under the control of LacI_I12 repressor

BBa_K116523
BBa_K116523 Version 1 (Component)
GFP under control of RpaA activated pOmpC promoter

BBa_K116520
BBa_K116520 Version 1 (Component)
GFP under control of RpaA activated pOmpF promoter

BBa_M1014
BBa_M1014 Version 1 (Component)
Complete construct for phasin secretion together with zinc finger binding sites

BBa_K1956012
BBa_K1956012 Version 1 (Component)
gRNA for mCherry gene under lambda pL hybrid promoter with terminator

BBa_K1956011
BBa_K1956011 Version 1 (Component)
gRNA for Chloramphenicol gene under lambda pL hybrid promoter with terminator

BBa_K606027
BBa_K606027 Version 1 (Component)
pVeg-SpoVG-YFP-tetR-Term for B. subtilis and E. coli

BBa_K2184038
BBa_K2184038 Version 1 (Component)
mutant SNP for non taster Umami & Monosodium gluten taste with guidRNA

BBa_M36667
BBa_M36667 Version 1 (Component)
FNR-dependent Hypoxia Sensor

BBa_M36661
BBa_M36661 Version 1 (Component)
E. coli fur protein

BBa_K415117
BBa_K415117 Version 1 (Component)
RBS Weak : pVIII-Fos

BBa_K1428000
BBa_K1428000 Version 1 (Component)
T7 promoter-driven BMC structural genes with a His6-tagged component for purification

BBa_K541028
BBa_K541028 Version 1 (Component)
Reflectin 1A (TAT-Constitutive) and LALF (TAT-IPTG inducable) protein for E.coli

BBa_J119394
BBa_J119394 Version 1 (Component)
tClone TetA+SacB: Device for Testing Transciptional Terminators and Riboswitches via GGA

BBa_K802004
BBa_K802004 Version 1 (Component)
Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>

BBa_K1437008
BBa_K1437008 Version 1 (Component)
mouse AhR detective protein 521-805 without terminator codon optimized for yeast

BBa_K1437006
BBa_K1437006 Version 1 (Component)
mouse AhR detective protein 83-805 with terminator codon optimized for yeast

BBa_J119367
BBa_J119367 Version 1 (Component)
tClone: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

BBa_K2071953
BBa_K2071953 Version 1 (Component)
A non-specific phosphotransferase, codon optimized for B.subtilis. Characterized in https://microbia

BBa_K1437007
BBa_K1437007 Version 1 (Component)
mouse AhR detective protein 521-805 with terminator codon optimized for yeast

BBa_K1179011
BBa_K1179011 Version 1 (Component)
eGFP (Green) fluorescent protein with a fused PEST site for rapid degradation