BBa_K777007BBa_K777007 Version 1 (Component)Tar receptor under the control of constitutive promoter J23113
BBa_K777005BBa_K777005 Version 1 (Component)Tar receptor under the control of constitutive promoter J23109
BBa_K777003BBa_K777003 Version 1 (Component)Tar receptor under the control of constitutive promoter J23105
BBa_K777006BBa_K777006 Version 1 (Component)Tar receptor under the control of constitutive promoter J23112
BBa_K777004BBa_K777004 Version 1 (Component)Tar receptor under the control of constitutive promoter J23106
BBa_K777103BBa_K777103 Version 1 (Component)flhDC operon under the control of constitutive promoter J23105
BBa_K346033BBa_K346033 Version 1 (Component)T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of mercury binding peptide)
BBa_I1070BBa_I1070 Version 1 (Component)XOR test/debug module
BBa_K2057004BBa_K2057004 Version 1 (Component)LasR under the regulation of the promoter BtI-BtII
BBa_K525582BBa_K525582 Version 1 (Component)Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic
BBa_K260018BBa_K260018 Version 1 (Component)Cell free Chassis: Mass production of aqueous vesicles in oil phase
BBa_K1326006BBa_K1326006 Version 1 (Component)Plac + POR + DVR1
BBa_G0007BBa_G0007 Version 1 (Component)Short sequence to promoter addition of 3' A overhang during PCR
BBa_G0008BBa_G0008 Version 1 (Component)Short sequence to promoter addition of 3' A overhang during PCR
BBa_K2151003BBa_K2151003 Version 1 (Component)pHLBA-E5501: GFP under control of strong constitutive promoter
BBa_K215210BBa_K215210 Version 1 (Component)IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin
BBa_K2151004BBa_K2151004 Version 1 (Component)p25-E5501: GFP under control of medium constitutive promoter
BBa_K2151005BBa_K2151005 Version 1 (Component)p32-E5501: GFP under control of strong constitutive promoter
BBa_I20243BBa_I20243 Version 1 (Component)Autoregulated synthesis of T7 RNAP with lacI negative feedback
BBa_K195406BBa_K195406 Version 1 (Component)This part is composed of F10scFv and C-IgAP
Scaffold gBBa_K1602029 Version 1 (Component)Inducible generator of protein scaffold with N-terminal HisTag
BBa_K831007BBa_K831007 Version 1 (Component)Inducible MqsR toxin under the control of tetR promoter
BBa_K831006BBa_K831006 Version 1 (Component)Inducible MqsR toxin under the control of tetR promoter
BBa_K1465105BBa_K1465105 Version 1 (Component)Fumarate reductase (Fum) under control of the T7 promoter
BBa_K2082117BBa_K2082117 Version 1 (Component)Six mutator genes under control of a modified Pbad
BBa_K831015BBa_K831015 Version 1 (Component)Inducible HipA7 toxin under the control of prm promoter
BBa_K215211BBa_K215211 Version 1 (Component)IPTG Inducible 5tmr OmpA Surface Display of WT Streptavidin
BBa_K091213BBa_K091213 Version 1 (Component)LacI X86 and pLac promoter upstream of GFP
BBa_K091210BBa_K091210 Version 1 (Component)lacIQ1 promoter under the control of LacI repressor
BBa_K091211BBa_K091211 Version 1 (Component)lacIQ promoter under the control of LacI_I12 repressor
BBa_K116523BBa_K116523 Version 1 (Component)GFP under control of RpaA activated pOmpC promoter
BBa_K116520BBa_K116520 Version 1 (Component)GFP under control of RpaA activated pOmpF promoter
SwitchBBa_K1185000 Version 1 (Component)B.subtilis Rod to L-form Switch
BBa_M36667BBa_M36667 Version 1 (Component)FNR-dependent Hypoxia Sensor
BBa_M36661BBa_M36661 Version 1 (Component)E. coli fur protein
BBa_K415117BBa_K415117 Version 1 (Component)RBS Weak : pVIII-Fos
BBa_M1014BBa_M1014 Version 1 (Component)Complete construct for phasin secretion together with zinc finger binding sites
BBa_K1956012BBa_K1956012 Version 1 (Component)gRNA for mCherry gene under lambda pL hybrid promoter with terminator
BBa_K1956011BBa_K1956011 Version 1 (Component)gRNA for Chloramphenicol gene under lambda pL hybrid promoter with terminator
BBa_K606027BBa_K606027 Version 1 (Component)pVeg-SpoVG-YFP-tetR-Term for B. subtilis and E. coli
BBa_K2184038BBa_K2184038 Version 1 (Component)mutant SNP for non taster Umami & Monosodium gluten taste with guidRNA
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K1428000BBa_K1428000 Version 1 (Component)T7 promoter-driven BMC structural genes with a His6-tagged component for purification
BBa_K541028BBa_K541028 Version 1 (Component)Reflectin 1A (TAT-Constitutive) and LALF (TAT-IPTG inducable) protein for E.coli
BBa_J119394BBa_J119394 Version 1 (Component)tClone TetA+SacB: Device for Testing Transciptional Terminators and Riboswitches via GGA
BBa_K802004BBa_K802004 Version 1 (Component)Shuttle vector for <i> E. coli</i> and <i>B. subtilis</i>
BBa_K1437008BBa_K1437008 Version 1 (Component)mouse AhR detective protein 521-805 without terminator codon optimized for yeast
BBa_K1437006BBa_K1437006 Version 1 (Component)mouse AhR detective protein 83-805 with terminator codon optimized for yeast
BBa_J119367BBa_J119367 Version 1 (Component)tClone: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly
BBa_K2071953BBa_K2071953 Version 1 (Component)A non-specific phosphotransferase, codon optimized for B.subtilis. Characterized in https://microbia