BBa_K584020
BBa_K584020 Version 1 (Component)
AFP extracellular protein generator

BBa_K1162307
BBa_K1162307 Version 1 (Component)
WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag

BBa_K2082207
BBa_K2082207 Version 1 (Component)
Fusion protein SH2:cMyc:cI(434)

BBa_P2005
BBa_P2005 Version 1 (Component)
Zif268-GCN4 protein generator

BBa_K1680002
BBa_K1680002 Version 1 (Component)
Protein linker, 12aa

BBa_K1680001
BBa_K1680001 Version 1 (Component)
protein linker, 9aa

BBa_K137115
BBa_K137115 Version 1 (Component)
Constitutively expresses cI, the lambda repressor.

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1361997
BBa_K1361997 Version 1 (Component)
CsgB, curlin nucleator protein, minor subunit in curli complex

BBa_M1175
BBa_M1175 Version 1 (Component)
protein1

creD
BBa_J64905 Version 1 (Component)
creD inner membrane protein

creC
BBa_J64906 Version 1 (Component)
creC/phoM protein kinase

BBa_J69516
BBa_J69516 Version 1 (Component)
lacI-regulated tetR invertor Protein Generator

BBa_K900004
BBa_K900004 Version 1 (Component)
Actin Targeting Protein

BBa_K247000
BBa_K247000 Version 1 (Component)
monomeric RFP: Red Fluorescent Protein.

BBa_K305004
BBa_K305004 Version 1 (Component)
Hydrophobic protein chaplin H (chpH)

BBa_K305003
BBa_K305003 Version 1 (Component)
Hydrophobic protein chaplin E (chpE)

BBa_C100003
BBa_C100003 Version 1 (Component)
ORF of the JMT odorant enzyme (PCR)

BBa_K543000
BBa_K543000 Version 1 (Component)
HNS protein (wild type)

BBa_J29028
BBa_J29028 Version 1 (Component)
rod shape-determining protein

BBa_J22146
BBa_J22146 Version 1 (Component)
The Lac Y and mRFP gene under the RecA(SOS) promoter.

BBa_K1390014
BBa_K1390014 Version 1 (Component)
mmoC-His

BBa_K1390016
BBa_K1390016 Version 1 (Component)
mmoX-His

BBa_K1390015
BBa_K1390015 Version 1 (Component)
mmoD-His

BBa_K1390018
BBa_K1390018 Version 1 (Component)
mmoZ-His

BBa_K1390013
BBa_K1390013 Version 1 (Component)
mmoB-His

Fos
BBa_K243027 Version 1 (Component)
His-FOS

BBa_K1390017
BBa_K1390017 Version 1 (Component)
mmoY-His

BBa_J97001
BBa_J97001 Version 1 (Component)
P-Free JuniperGFP (Green Fluorescent Protein)

BBa_K1510017
BBa_K1510017 Version 1 (Component)
red florescence protein RFC23

BBa_K1669003
BBa_K1669003 Version 1 (Component)
CtfB + His-tag

BBa_K1669004
BBa_K1669004 Version 1 (Component)
BdhB + His-tag

BBa_K1669001
BBa_K1669001 Version 1 (Component)
CtfA + His-tag

BBa_K427002
BBa_K427002 Version 1 (Component)
Mor protein of Mu bacteriophage

BBa_K1447006
BBa_K1447006 Version 1 (Component)
HIS-TEV

BBa_K500004
BBa_K500004 Version 1 (Component)
Display the Lip on the surface of yeast

BBa_K1165015
BBa_K1165015 Version 1 (Component)
SAM Synthetase under the Control of the pTet Promoter

BBa_K809723
BBa_K809723 Version 1 (Component)
GAL promoter + kozak + hoiln(lambda phage)+ ADH1 terminator

BBa_K809718
BBa_K809718 Version 1 (Component)
GAL promoter + ZIM17 + hoiln(lambda phage) + ADH1 terminator

BBa_K1583015
BBa_K1583015 Version 1 (Component)
Linker in fusion protein

BBa_K1149033
BBa_K1149033 Version 1 (Component)
pudA-his

BBa_I12017
BBa_I12017 Version 1 (Component)
Protein Generator (434, -LVA)

BBa_K327009
BBa_K327009 Version 1 (Component)
Las receiver

BBa_K801032
BBa_K801032 Version 1 (Component)
GAL4 DNA binding domain

BBa_I12038
BBa_I12038 Version 1 (Component)
Kickstart for the Barkai-Leibler oscillator

BBa_K1732024
BBa_K1732024 Version 1 (Component)
YFPCO-His

TraJR
BBa_J01001 Version 1 (Component)
TraJr protein (controls conjugative transfer in RP4 plasmid)

BBa_K1462800
BBa_K1462800 Version 1 (Component)
GAL1+GBD+SH3+SH3+SH3+PDZ+PDZ+PDZ+PDZ+ADH1