BBa_K1491033
BBa_K1491033 Version 1 (Component)
T7 promoter-RBS-RFP-T7 Terminator

BBa_J133000
BBa_J133000 Version 1 (Component)
RFP Reporter Cassette B0032.E1010.B0015

BBa_J133001
BBa_J133001 Version 1 (Component)
RFP Reporter Cassette B0034.E1010.B0015

BBa_J01098
BBa_J01098 Version 1 (Component)
TEST [Str RBS][RFP][Dbl Term]

BBa_K315023
BBa_K315023 Version 1 (Component)
pTet-loxBri(F)-RBS-RFP-loxBri(F)

BBa_I13972
BBa_I13972 Version 1 (Component)
Lux CFP Protein Generator (R0062.E0420)

BBa_I13971
BBa_I13971 Version 1 (Component)
c1 CFP Protein Generator (R0051.E0420)

BBa_J01119
BBa_J01119 Version 1 (Component)
TEST [pBad/araC][StrRBS][RFP][DblTerm]

BBa_K944007
BBa_K944007 Version 1 (Component)
Higly engineered mutant of RFP and a double terminator

BBa_K546547
BBa_K546547 Version 1 (Component)
Constitutive (tetR repressible) LacI and RFP expression

BBa_K264028
BBa_K264028 Version 1 (Component)
Constitutive promoter BBa_J23102/RFP reporter BBa_J06702

BBa_K086000
BBa_K086000 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter - YFP

BBa_S03767
BBa_S03767 Version 1 (Component)
T7Promoter-RBS-RFP1-HixC-GFP2 (pSB1A2)

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_S03766
BBa_S03766 Version 1 (Component)
RBS-Kan-RBS-Tet-RBS-RFP (pSB1A7)

BBa_K199170
BBa_K199170 Version 1 (Component)
T7 Promoter RBS RFP pLac CCACC (tRNA)

BBa_K199169
BBa_K199169 Version 1 (Component)
T7 Promoter RBS RFP pLac CUAGC (tRNA)

BBa_K371043
BBa_K371043 Version 1 (Component)
MPF(meta-prefix)+RFP+MSF(meta-suffix)

BBa_K110019
BBa_K110019 Version 1 (Component)
mCherry FP (Backwards)

BBa_K775012
BBa_K775012 Version 1 (Component)
diacetyl chimeric GPCR with Fus1-EGFP reporter (2)

BBa_K2123111
BBa_K2123111 Version 1 (Component)
Tac promoter with downstream mer operator + RFP (K081014)

BBa_S03644
BBa_S03644 Version 1 (Component)
RFP_rev : RBS_rev

BBa_J70459
BBa_J70459 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0031 simulator (reverse oligo)

BBa_K1923007
BBa_K1923007 Version 1 (Component)
Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

BBa_K101013
BBa_K101013 Version 1 (Component)
Construct with TetR/p22cII promoter, RBS, RFP, Terminator

BBa_K2123113
BBa_K2123113 Version 1 (Component)
Stationary phase promoter with downstream mer operator + RFP (K081014)

BBa_J162001
BBa_J162001 Version 1 (Component)
RBS cl RBS RFP RBS 3OC6HSL Double Terminator

BBa_K101012
BBa_K101012 Version 1 (Component)
Construct with TetR/p22cII promoter, RBS, RFP, Terminator

BBa_I13603
BBa_I13603 Version 1 (Component)
Lac operator with CFP reporter (with LVA tag) [R/Lc+]

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_K092500
BBa_K092500 Version 1 (Component)
TetR cds with RBS and Terminator + pTetR, RFP with RBS

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K2123114
BBa_K2123114 Version 1 (Component)
Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K2123116
BBa_K2123116 Version 1 (Component)
Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.