BBa_K1361004BBa_K1361004 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag
BBa_K079052BBa_K079052 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
BBa_K079020BBa_K079020 Version 1 (Component)GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
BBa_K332034BBa_K332034 Version 1 (Component)Constitutive promoter + 37℃ induced RBS + tetR + double terminator
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
BBa_K887004BBa_K887004 Version 1 (Component)Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015
BBa_K987001BBa_K987001 Version 1 (Component)This is a composite part which has the function to invert the temperature activation by the part: BB
BBa_J04430BBa_J04430 Version 1 (Component)GFP coding device switched on by IPTG
BBa_K843003BBa_K843003 Version 1 (Component)Lambda cI regulated by PLacI
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K886002BBa_K886002 Version 1 (Component)Recombination Device composed by Cre-lox71
PL GFPBBa_K193000 Version 1 (Component)GFP reporter regulated by CI.
pTetR-LacIBBa_I763030 Version 1 (Component)LacI coding device regulated by pTetR
BBa_K726009BBa_K726009 Version 1 (Component)T7 driven lac operated inducer for the rhl quorum-sensing system
BBa_K1321362BBa_K1321362 Version 1 (Component)sfGFP fused to CBDcex driven by LacI
BBa_I763003BBa_I763003 Version 1 (Component)GFP coding device switched on by IPTG
BBa_K1615108BBa_K1615108 Version 1 (Component)mRFP fused to CBDclos driven by LacI promoter
PLac-LacY-BBa_I763013 Version 1 (Component)LacY and cI coding device switched on by IPTG
BBa_K258003BBa_K258003 Version 1 (Component)Granulysin, a T Cell Product,Kills Bacteria by Altering Membrane Permeability
BBa_K395102BBa_K395102 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)
BBa_K395103BBa_K395103 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)
BBa_J58014BBa_J58014 Version 1 (Component)Promoter activated by OmpR-P with the reporter GFP
BBa_K290001BBa_K290001 Version 1 (Component)constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl
BBa_J58015BBa_J58015 Version 1 (Component)Mutated promoter activated by OmpR-P with the reporter GFP
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_K300096BBa_K300096 Version 1 (Component)Double phasin and intein separed by a flexible protein domain linker
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K2144011BBa_K2144011 Version 1 (Component)Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.