SspDnaB(N)
BBa_K1362408 Version 1 (Component)
SspDnaB N-Intein cloning piece

BBa_K1859012
BBa_K1859012 Version 1 (Component)
2*GSGSGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859010
BBa_K1859010 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*3 linker

BBa_K1859009
BBa_K1859009 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*3 linker

BBa_K1859014
BBa_K1859014 Version 1 (Component)
3*GSGSGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859007
BBa_K1859007 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GGSGGS*2 linker

BBa_K1859011
BBa_K1859011 Version 1 (Component)
2*GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

BBa_K1859008
BBa_K1859008 Version 1 (Component)
the 3??side of the intron[BBa_K1332005] +GSGSGS*2 linker

BBa_K1859013
BBa_K1859013 Version 1 (Component)
3*GGSGGS linker+ the 5??side of the intron[BBa_K1332003]

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

BBa_K1903019
BBa_K1903019 Version 1 (Component)
Intein N SSp DnaB S1

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

BBa_K1051974
BBa_K1051974 Version 1 (Component)
clb6 P + D-box + GFP + MER2 Intron + TOM40 + T

BBa_J32009
BBa_J32009 Version 1 (Component)
4HT Dependent Intein

TerDnaE3(N
BBa_K1362404 Version 1 (Component)
TerDnaE3 N-Intein cloning piece

SspGyrB(N)
BBa_K1362406 Version 1 (Component)
SspGyrB N-Intein cloning piece

SspDnaX(N)
BBa_K1362402 Version 1 (Component)
SspDnaX N-Intein cloning piece

TerDnaE3(C
BBa_K1362405 Version 1 (Component)
TerDnaE3 C-Intein cloning piece

SspGyrB(C)
BBa_K1362407 Version 1 (Component)
SspGyrB C-Intein cloning piece

Gp41-1(N)
BBa_K1362412 Version 1 (Component)
Gp41-1 N-Intein cloning piece

SspDnaX(C)
BBa_K1362403 Version 1 (Component)
SspDnaX C-Intein cloning piece

BBa_K382066
BBa_K382066 Version 1 (Component)
intron hairpin RNAi knockdown of Ger allergen in Basta plant vector

BBa_K382070
BBa_K382070 Version 1 (Component)
intron hairpin RNAi knockdown of LTP allergen in Basta plant vector

BBa_K382067
BBa_K382067 Version 1 (Component)
intron hairpin RNAi knockdown of Ger allergen in Kan plant vector

BBa_K382071
BBa_K382071 Version 1 (Component)
intron hairpin RNAi knockdown of LTP allergen in Kan plant vector

NpuDnaE(C)
BBa_K1362479 Version 1 (Component)
nonsplicing NpuDnaE C-Intein cloning piece

BBa_K1465230
BBa_K1465230 Version 1 (Component)
T7-RBS Intein-tag

TerDnaE3(N
BBa_K1362130 Version 1 (Component)
RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)

SspDnaB(N)
BBa_K1362140 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] assembly construct (with His6)

SspDnaE(N)
BBa_K1362410 Version 1 (Component)
SspDnaE N-Intein cloning piece

1NpuE(N*)
BBa_K1362102 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)

TerDnaE3(C
BBa_K1362131 Version 1 (Component)
RBS + TerDnaE3 C-intein RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362141 Version 1 (Component)
RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)

SspDnaX(N)
BBa_K1362110 Version 1 (Component)
RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)

SspGyrB(N)
BBa_K1362120 Version 1 (Component)
RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)

SspGyrB(C)
BBa_K1362121 Version 1 (Component)
RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)

SspDnaE(C)
BBa_K1362411 Version 1 (Component)
SspDnaE C-Intein cloning piece

BBa_J48103
BBa_J48103 Version 1 (Component)
Iron promoter

1NpuE(C*)
BBa_K1362103 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)

BBa_K1362111
BBa_K1362111 Version 1 (Component)
RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)

Gp41-1(C)
BBa_K1362413 Version 1 (Component)
Gp41-1 C-Intein cloning piece

BBa_K1332005
BBa_K1332005 Version 1 (Component)
The 3' side of the intron(+exon fragment) from td gene of T4 phage

BBa_K1491025
BBa_K1491025 Version 1 (Component)
T7 RNA Polymerase with ER Intein

BBa_K1916011
BBa_K1916011 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916022
BBa_K1916022 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916005
BBa_K1916005 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916008
BBa_K1916008 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag