CircNpu

BBa_K1362000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1362000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:04

NpuDnaE intein RFC[105] circularization construct

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1362000_sequence (Version 1)

Description

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Notes

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Source

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Components

Access	Instance	Definition
public	BBa_K1362090	T7 RBS
public	BBa_G0000	scar
public	BBa_K1362414	RFC105 A
public	BBa_K1362401	NpuDnaE(C)
public	BBa_K1362419	RFC105 CC
public	BBa_K1362423	<- BsaI
public	BBa_K1362505	BBa_04450*
public	BBa_K1362424	BsaI ->
public	BBa_K1362415	RFC105 NN
public	BBa_K1362400	NpuDnaE(N)
public	BBa_K1362421	RFC105 Z
public	BBa_K1362999	BBa_K1362999

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1362090	1,29	T7 RBS
BBa_G0000	30,35	scar
BBa_K1362414	36,38	RFC105 A
BBa_K1362401	39,139	NpuDnaE(C)
BBa_K1362419	140,143	RFC105 CC
BBa_K1362423	144,150	<- BsaI
BBa_K1362505	151,1219	BBa_04450*
BBa_K1362424	1220,1226	BsaI ->
BBa_K1362415	1227,1230	RFC105 NN
BBa_K1362400	1231,1532	NpuDnaE(N)
BBa_K1362421	1533,1536	RFC105 Z
BBa_K1362999	1537,1562	BBa_K1362999

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1362000/1

Member of these Collections

• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 21K
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry