BBa_J06611
BBa_J06611 Version 1 (Component)
Construction intermediate: LacI ts (mut 241) QPI with Lambda cI regulated promoter (R0051.J06800)

BBa_K1766008
BBa_K1766008 Version 1 (Component)
EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>.

BBa_K1766014
BBa_K1766014 Version 1 (Component)
EnvZ osmoregulatory histidine kinase from <i>E.coli.</i>

BBa_K112211
BBa_K112211 Version 1 (Component)
{a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format

BBa_K133005
BBa_K133005 Version 1 (Component)
CF-UreB-RGD-Histop

BBa_K133039
BBa_K133039 Version 1 (Component)
T7-CF-UREB-RGD-Histop

BBa_K242999
BBa_K242999 Version 1 (Component)
Colicin E3 rRNAse domain

BBa_K2129001
BBa_K2129001 Version 1 (Component)
EutS compartment-forming protein

BBa_K133037
BBa_K133037 Version 1 (Component)
T7-CF213-UB33-CF215-RGD-Histop

BBa_K606046
BBa_K606046 Version 1 (Component)
KinA, sporulation gene for B. Subtilis

BBa_K133020
BBa_K133020 Version 1 (Component)
CMV-CF213-multiHP-CF215-RGD-Histop (term.)

BBa_K809603
BBa_K809603 Version 1 (Component)
Bovine Pancreatic DNase I (in yeast mt codon table)

BBa_K133023
BBa_K133023 Version 1 (Component)
CMV-ss-CF213-multiHP-CF215-RGD-Histop (term)

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_J24822
BBa_J24822 Version 1 (Component)
Same as J24819 but with the error at the luc-terminator junction fixed

BBa_K2066500
BBa_K2066500 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.