BBa_K1650043
BBa_K1650043 Version 1 (Component)
ADH II

BBa_K1049002
BBa_K1049002 Version 1 (Component)
Isoamyl acetate generator

EYFP
BBa_E0036 Version 1 (Component)
enhanced yellow fluorescent protein derived from A. victoria GFP

ECFP
BBa_E0026 Version 1 (Component)
enhanced cyan fluorescent protein derived from A. victoria GFP

BBa_K918000
BBa_K918000 Version 1 (Component)
Mating Factor Alpha Secretion Tag

BBa_J23150
BBa_J23150 Version 1 (Component)
1bp mutant from J23107

BBa_J07009
BBa_J07009 Version 1 (Component)
toxicity-gene activator from Vibrio cholerae

BBa_I732083
BBa_I732083 Version 1 (Component)
[aTc] -> PoPS

PrtDEF
BBa_K258008 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_I737001
BBa_I737001 Version 1 (Component)
protein generator for XXXX for making YYYY

GRE3
BBa_K1602004 Version 1 (Component)
Aldose reductase (GRE3) from Saccharomyces cerevisiae S288c

BBa_K316003
BBa_K316003 Version 1 (Component)
XylE - catechol 2,3-dioxygenase from P.putida with terminator

FUS1 pr
BBa_K1154001 Version 1 (Component)
Saccharomyces cerevisiae FUS1 promoter (induced by mating pheromone)

ClpX
BBa_K365004 Version 1 (Component)
ClpX from E.coli (aa 61-425)

BBa_K112406
BBa_K112406 Version 1 (Component)
cadC promoter

BBa_K776023
BBa_K776023 Version 1 (Component)
EAB ABC

BBa_K638001
BBa_K638001 Version 1 (Component)
Reflectin A1 from Loligo

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

BBa_K1499502
BBa_K1499502 Version 1 (Component)
Cellulose Acetate Esterase (Cae)

BBa_I746365
BBa_I746365 Version 1 (Component)
PLL promoter from P4 phage

BBa_K289005
BBa_K289005 Version 1 (Component)
PDC

victor
BBa_I714005 Version 1 (Component)
abc

BBa_K137006
BBa_K137006 Version 1 (Component)
pabB (from pABA synthesis)

BBa_B0023
BBa_B0023 Version 1 (Component)
TE from coliphage T7, reversed

BBa_I11031
BBa_I11031 Version 1 (Component)
excisionase from E. coli phage P22 (removes prophage from host genome)

BBa_K177026
BBa_K177026 Version 1 (Component)
listeriolysin O 399G->C - locus from Listeria monocytogenes

BBa_K118019
BBa_K118019 Version 1 (Component)
Plac+rbs+glgC (ADP-glucose pyrophosphorylase)

BBa_K797000
BBa_K797000 Version 1 (Component)
tatABCD from E.coli

BBa_K2146004
BBa_K2146004 Version 1 (Component)
pWH1266 ori-Ptac-lacOc-ampC ADC-33-pSB1C3

pabA
BBa_K137005 Version 1 (Component)
pabA (from pABA synthesis)

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_K218001
BBa_K218001 Version 1 (Component)
LuxOU from Vibrio harveyi

BBa_M36038
BBa_M36038 Version 1 (Component)
PoPS->Alpha Factor Mating Pheromone

BBa_K863021
BBa_K863021 Version 1 (Component)
bhal laccase from Bacillus halodurans

oprF
BBa_K1172501 Version 1 (Component)
Outer membrane porin OprF from Pseudomonas fluorescens

BBa_K173011
BBa_K173011 Version 1 (Component)
aTc inducible system (aTc->PoPS) with J23118 promoter

phlF
BBa_K1725040 Version 1 (Component)
<i>phlF</i> encoding PhlF repressor from Pseudomonas protegens Pf-5

BBa_I11021
BBa_I11021 Version 1 (Component)
excisionase from E. coli phage lambda (removes prophage from host genome)

BBa_K896902
BBa_K896902 Version 1 (Component)
Against aging!!

Ptet-ChiX
BBa_K564014 Version 1 (Component)
atC-->chiX

BBa_K1172901
BBa_K1172901 Version 1 (Component)
Alanine racemase from ''E. coli''

BBa_K106671
BBa_K106671 Version 1 (Component)
GFP, Aar1 AD part

Pveg
BBa_K316001 Version 1 (Component)
pVeg Constitutive promoter for Veg locus from B. subtilis

BBa_K863001
BBa_K863001 Version 1 (Component)
bpul laccase from Bacillus pumilus

BBa_K863006
BBa_K863006 Version 1 (Component)
ecol laccase from E. coli

BBa_K863011
BBa_K863011 Version 1 (Component)
tthl laccase from Thermus thermophilus