BBa_J100242
BBa_J100242 Version 1 (Component)
mdtG Promotor in the Marbox Regulon Induced by Paraquat/Asprin

BBa_K1334016
BBa_K1334016 Version 1 (Component)
formate dehydrogenase(FDH) from Escherichia.coli DH5a

BBa_K1470002
BBa_K1470002 Version 1 (Component)
Galactose-induced gene 4 DNA binding domain (Gal4DBD)

BBa_K112324
BBa_K112324 Version 1 (Component)
{< phoA!}{< b1006 >} in BBb

BBa_K1319014
BBa_K1319014 Version 1 (Component)
Constitutive expression of GFP-REACh2 fusion protein

BBa_K1319015
BBa_K1319015 Version 1 (Component)
Constitutive expression of GFP-EYFP fusion protein

BBa_K1319013
BBa_K1319013 Version 1 (Component)
Constitutive expression of GFP-REACh1 fusion protein

BBa_K2037010
BBa_K2037010 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037014
BBa_K2037014 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037005
BBa_K2037005 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037016
BBa_K2037016 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037009
BBa_K2037009 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037007
BBa_K2037007 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037015
BBa_K2037015 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037013
BBa_K2037013 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037008
BBa_K2037008 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037012
BBa_K2037012 Version 1 (Component)
Laccase For in vitro expression

BBa_K2037006
BBa_K2037006 Version 1 (Component)
Laccase for in vitro expression

BBa_K2037017
BBa_K2037017 Version 1 (Component)
Laccase for in vitro expression

BBa_K1774001
BBa_K1774001 Version 1 (Component)
Cas9 (optimized for expression in E. coli)

BBa_I722008
BBa_I722008 Version 1 (Component)
GFP Producer induced by IPTG

BBa_K950001
BBa_K950001 Version 1 (Component)
yeast rox1

ADH
BBa_M36200 Version 1 (Component)
ADH; converts ethanol to/from acetaldehyde; no STOP codon

BBa_K376012
BBa_K376012 Version 1 (Component)
Constitutive LuxR generator

BBa_K376013
BBa_K376013 Version 1 (Component)
Constitutive LuxR generator

BBa_K218002
BBa_K218002 Version 1 (Component)
LuxOU under constitutive expression of TetR repressible promoter

BBa_K957005
BBa_K957005 Version 1 (Component)
Constitutive expression of nah Operon for degradation of naphthalene to salicylate

BBa_K1559005
BBa_K1559005 Version 1 (Component)
CRISPR/Cas9 system with Anderson low-expression constitutive promoter

BBa_K1559003
BBa_K1559003 Version 1 (Component)
CRISPR/Cas9 system with Anderson high-expression constitutive promoter

BBa_K1559004
BBa_K1559004 Version 1 (Component)
CRISPR/Cas9 system with Anderson medium-expression constitutive promoter

BBa_J100235
BBa_J100235 Version 1 (Component)
Arabinose induced araBAD

BBa_J100230
BBa_J100230 Version 1 (Component)
HFQp:Cold-induced promoter

BBa_K1650001
BBa_K1650001 Version 1 (Component)
Constitutive promoter expressing GFP (ILS 2015)

BBa_K1650012
BBa_K1650012 Version 1 (Component)
Constitutive promoter expressing GFP (ILS 2015)

BBa_K1650002
BBa_K1650002 Version 1 (Component)
Constitutive promoter expressing GFP (ILS 2015)

BBa_J72009
BBa_J72009 Version 1 (Component)
pBca1101 RFP expression plasmid in BBb

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1871002
BBa_K1871002 Version 1 (Component)
Alpha amylase with mating factor alpha secretion

BBa_K1403013
BBa_K1403013 Version 1 (Component)
Geosmin expression cassette

BBa_K590030
BBa_K590030 Version 1 (Component)
DrR-FabH2-PSB1C3-High constitutive

P43
BBa_K143013 Version 1 (Component)
Promoter 43 a constitutive promoter for B. subtilis

HMG-CoA-R
BBa_K849000 Version 1 (Component)
3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast

BBa_K360128
BBa_K360128 Version 1 (Component)
J23114 constitutive promoter + LovTAP Photosensor

BBa_K360126
BBa_K360126 Version 1 (Component)
J23102 constitutive promoter + LovTAP Photosensor

BBa_K360129
BBa_K360129 Version 1 (Component)
J23117 constitutive promoter + LovTAP Photosensor

BBa_K360127
BBa_K360127 Version 1 (Component)
J23105 constitutive promoter + LovTAP Photosensor

BBa_K142075
BBa_K142075 Version 1 (Component)
lac-controlled CFP generator with constitutive lacI expression cassette

BBa_K142076
BBa_K142076 Version 1 (Component)
lac-controlled RFP generator with constitutive lacI expression cassette

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette