BBa_J70501
BBa_J70501 Version 1 (Component)
Plasmid origin from pWV01 + Tet resistance gene for plasmid construction

BBa_K759003
BBa_K759003 Version 1 (Component)
phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_J72210
BBa_J72210 Version 1 (Component)
Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site

BBa_K2116000
BBa_K2116000 Version 1 (Component)
RBS designed to achieve maximal expression of EsaR

BBa_J100237
BBa_J100237 Version 1 (Component)
Original Nitrate Biosensor (O)

BBa_K1362013
BBa_K1362013 Version 1 (Component)
RBS + Circular λ-Lysozyme with rigid linker

BBa_K759006
BBa_K759006 Version 1 (Component)
B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_J10068
BBa_J10068 Version 1 (Component)
luciferase + LRE + BD18 original RBS removed

BBa_K806003
BBa_K806003 Version 1 (Component)
SeqA regulation of chromosome replication by preventing re-initiation at newly replicated origins

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

BBa_J119405
BBa_J119405 Version 1 (Component)
(pSB1A2-BR) Deletion of the original -35 sequence

BBa_K809105
BBa_K809105 Version 1 (Component)
Original termination efficiency test BioBricks for Tq0255

BBa_K809104
BBa_K809104 Version 1 (Component)
Original termination efficiency test BioBricks for Tcox3

BBa_K809103
BBa_K809103 Version 1 (Component)
Original termination efficiency test BioBricks for Tcob

BBa_J56016
BBa_J56016 Version 1 (Component)
Rbs-orig - ribosome binding site

BBa_K563031
BBa_K563031 Version 1 (Component)
pEASY with first part of Tor2 without a original promoter

BBa_K1937002
BBa_K1937002 Version 1 (Component)
OriKan: a composite part to turn any pSB1C3 into a <i>Bacillus</i> plasmid

BBa_K1413041
BBa_K1413041 Version 1 (Component)
Mutation of OriVR6Kgamma origin of replication (ori)

BBa_J72003
BBa_J72003 Version 1 (Component)
{R6K} conditional origin of replication in BBb

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.