BBa_K782024BBa_K782024 Version 1 (Component)2x[NicTAL]+2x[TALD] operator_minimal promoter_mCitrine
BBa_K782025BBa_K782025 Version 1 (Component)4x[NicTAL]+4x[TALD] operator_minimal promoter_mCitrine
BBa_K782026BBa_K782026 Version 1 (Component)7x[TALA+TALB] operator_minimal promoter_mCitrine
BBa_K782027BBa_K782027 Version 1 (Component)7x[TALA] operator_minimal promoter_mCitrine
BBa_K782028BBa_K782028 Version 1 (Component)10x[TALA+TALB] operator_minimal promoter_mCitrine
BBa_K782029BBa_K782029 Version 1 (Component)10x[TALA] operator_minimal promoter_mCitrine
BBa_K759007BBa_K759007 Version 1 (Component)RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)
mtGFPBBa_K809001 Version 1 (Component)Mitochondrial GFP
BBa_K782030BBa_K782030 Version 1 (Component)10x[TALB] operator_minimal promoter_mCitrine
BBa_K782031BBa_K782031 Version 1 (Component)12x[TALD] operator_minimal promoter_mCitrine
nitRBBa_K836007 Version 1 (Component)Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)
BBa_K782032BBa_K782032 Version 1 (Component)10x[TALA+TALB] operator_minimal promoter_fLuciferase
BBa_K782033BBa_K782033 Version 1 (Component)10x[TALA] operator_minimal promoter_fLuciferase
BBa_K1982002BBa_K1982002 Version 1 (Component)Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K113004BBa_K113004 Version 1 (Component)pT7->tetR operator->GFP
BBa_S04136BBa_S04136 Version 1 (Component)terminator and cI promoter
BBa_K098996BBa_K098996 Version 1 (Component)mtrB with terminator
BBa_K113005BBa_K113005 Version 1 (Component)pLac->tetR->pT7->tetR operator ->GFP
BBa_K098994BBa_K098994 Version 1 (Component)mtrB with RBS and terminator
BBa_K105020BBa_K105020 Version 1 (Component)tetR - operator
BBa_K105021BBa_K105021 Version 1 (Component)cI - operator
BBa_K105022BBa_K105022 Version 1 (Component)lex A - operator
BBa_K105023BBa_K105023 Version 1 (Component)lac I - operator
BBa_K105024BBa_K105024 Version 1 (Component)Gal4 - operator
BBa_K105025BBa_K105025 Version 1 (Component)Adh1 terminator
BBa_K157002BBa_K157002 Version 1 (Component)Transmembrane region of the EGF-Receptor (ErbB-1)
BBa_K113021BBa_K113021 Version 1 (Component)Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter
BBa_K157010BBa_K157010 Version 1 (Component)glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_K143067BBa_K143067 Version 1 (Component)AmyE integratable PoPS generator (P43-gsiB)
BBa_K143068BBa_K143068 Version 1 (Component)AmyE integratable PoPS generator (P43-spoVG)
BBa_K143069BBa_K143069 Version 1 (Component)AmyE integratable PoPS generator (Pveg-gsiB)
EukaryoticBBa_K157040 Version 1 (Component)Eukaryotic terminator vector (very similar to pSB1AK8)
TransfectiBBa_K157041 Version 1 (Component)Transfection vector (similar to pSB1AK8)
BBa_K102951BBa_K102951 Version 1 (Component)TA1In anti-sense input to TA1 (BBa_K102901)
BBa_K102952BBa_K102952 Version 1 (Component)TA2In anti-sense input to BBa_K102952
BBa_K102953BBa_K102953 Version 1 (Component)TA13n anti-sense input to TA3 (BBa_K102903)
BBa_K102954BBa_K102954 Version 1 (Component)TA6In anti-sense input to BBa_K102904
BBa_K102955BBa_K102955 Version 1 (Component)TA7In anti-sense input to BBa_K102905
BBa_K102956BBa_K102956 Version 1 (Component)TA8In anti-sense input to BBa_K102906
BBa_K102957BBa_K102957 Version 1 (Component)TA9In anti-sense input to BBa_K102907
BBa_K102958BBa_K102958 Version 1 (Component)TA10In anti-sense input to BBa_K102908
BBa_K102959BBa_K102959 Version 1 (Component)TA11In anti-sense input to BBa_K102909
BBa_K102960BBa_K102960 Version 1 (Component)TA12In anti-sense input to anti-terminator BBa_K102910
BBa_K102961BBa_K102961 Version 1 (Component)TA13In anti-sense input to BBa_K102911
BBa_K102962BBa_K102962 Version 1 (Component)TA14In anti-sense input to BBa_K102912
BBa_K143070BBa_K143070 Version 1 (Component)AmyE integratable PoPS generator (Pveg-spoVG)