BBa_I756028
BBa_I756028 Version 1 (Component)
cmv - IgM signal- VH-VL-EporD2-EporTm mut- gp130i- dnaEC-VP16-SV40PA

BBa_K2066112
BBa_K2066112 Version 1 (Component)
Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard

BBa_K769018
BBa_K769018 Version 1 (Component)
Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CABFEG-DT

BBa_K769017
BBa_K769017 Version 1 (Component)
Promoter(Pbad)-RBS-luxA-DT-Promoter(high)-RBS-lux CDBFEG-DT

BBa_J06911
BBa_J06911 Version 1 (Component)
LacI ts (mut 241) QPI regulated by lambda cI with EYFP

BBa_J06912
BBa_J06912 Version 1 (Component)
LacI ts (mut 265) QPI regulated by lambda cI with EYFP

BBa_J06902
BBa_J06902 Version 1 (Component)
LacI ts (mut 265) QPI regulated by lambda cI with mCherry

BBa_J06901
BBa_J06901 Version 1 (Component)
LacI ts (mut 241) QPI regulated by lambda cI with mCherry

BBa_J06961
BBa_J06961 Version 1 (Component)
Lambda cI/LacI ts (mut 241) RS flip-flop with EYFP

BBa_J06962
BBa_J06962 Version 1 (Component)
Lambda cI/LacI ts (mut 265) RS flip-flop with EYFP

BBa_K1014999
BBa_K1014999 Version 1 (Component)
Biological proportional operational Mu-circuit(1)

BBa_K658014
BBa_K658014 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-5 Driven by PLlac 0-1

BBa_K658013
BBa_K658013 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-3 Driven by PLlac 0-1

BBa_K180006
BBa_K180006 Version 1 (Component)
Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]

BBa_K658015
BBa_K658015 Version 1 (Component)
3OC6HSL Sender and Receiver Device with lux pR-3/5 Driven by PLlac 0-1

BBa_K1497197
BBa_K1497197 Version 1 (Component)
B0034-CHI - Chalcone Isomerase from Petunia with strong RBS

BBa_J147001
BBa_J147001 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_J147000
BBa_J147000 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_J147002
BBa_J147002 Version 1 (Component)
RFP inducible device regulated by LuxR and C6-HSL through the Lux promoter

BBa_K658010
BBa_K658010 Version 1 (Component)
a bacteria population-control device with lux pR-5 driven by lacl+pL

BBa_K658009
BBa_K658009 Version 1 (Component)
a bacteria population-control device with lux pR-3 driven by lacl+pL

BBa_J06612
BBa_J06612 Version 1 (Component)
Construction intermediate: LacI ts (mut 265) QPI with Lambda cI regulated promoter (R0051.J06801)

BBa_J06611
BBa_J06611 Version 1 (Component)
Construction intermediate: LacI ts (mut 241) QPI with Lambda cI regulated promoter (R0051.J06800)

BBa_K176116
BBa_K176116 Version 1 (Component)
Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)

BBa_K427008
BBa_K427008 Version 1 (Component)
Ter PBadweak Mu Mor Ter PM GFP cassette

BBa_K427007
BBa_K427007 Version 1 (Component)
Ter PBadweak Mu C Ter Pmom GFP cassette

BBa_K1633005
BBa_K1633005 Version 1 (Component)
MOR siRNA-3 (siRNA for mouse Mu opioid receptor)

BBa_K1633004
BBa_K1633004 Version 1 (Component)
MOR siRNA-2 (siRNA for mouse Mu opioid receptor)

BBa_K1633003
BBa_K1633003 Version 1 (Component)
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

BBa_K1633006
BBa_K1633006 Version 1 (Component)
MOR siRNA-4 (siRNA for mouse Mu opioid receptor)

BBa_K658011
BBa_K658011 Version 1 (Component)
a bacteria population-control device with lux pR-3/5 driven by lacl+pL

BBa_K176092
BBa_K176092 Version 1 (Component)
Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K188006
BBa_K188006 Version 1 (Component)
Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be

BBa_J28028
BBa_J28028 Version 1 (Component)
lux autoinducer

BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_K1541000
BBa_K1541000 Version 1 (Component)
promoter lux pR with riboregulator RR12y

BBa_K327015
BBa_K327015 Version 1 (Component)
Lux activated, C1lam repressed switch

BBa_J10064
BBa_J10064 Version 1 (Component)
Lux Operon Vibrio fischeri from part K325909

BBa_K1541025
BBa_K1541025 Version 1 (Component)
sfGFP under promoter P(Lux) with riboregulator RR12y

BBa_J329001
BBa_J329001 Version 1 (Component)
Simple lux-based QS circuit expressing untagged GFP

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_K584011
BBa_K584011 Version 1 (Component)
Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP

BBa_I13211
BBa_I13211 Version 1 (Component)
Biobricked version of the natural Lux quorum sensing system

BBa_K1014015
BBa_K1014015 Version 1 (Component)
Biological proportional operational Mu-circuit(1)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.