BBa_K1444004
BBa_K1444004 Version 1 (Component)
Composite promoter and weak <i>B. subtilis </i> RBS - <i>C1-&#955;</i>

BBa_J06612
BBa_J06612 Version 1 (Component)
Construction intermediate: LacI ts (mut 265) QPI with Lambda cI regulated promoter (R0051.J06801)

BBa_J06611
BBa_J06611 Version 1 (Component)
Construction intermediate: LacI ts (mut 241) QPI with Lambda cI regulated promoter (R0051.J06800)

BBa_K176116
BBa_K176116 Version 1 (Component)
Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)

BBa_K427008
BBa_K427008 Version 1 (Component)
Ter PBadweak Mu Mor Ter PM GFP cassette

BBa_K427007
BBa_K427007 Version 1 (Component)
Ter PBadweak Mu C Ter Pmom GFP cassette

BBa_K1633005
BBa_K1633005 Version 1 (Component)
MOR siRNA-3 (siRNA for mouse Mu opioid receptor)

BBa_K1633004
BBa_K1633004 Version 1 (Component)
MOR siRNA-2 (siRNA for mouse Mu opioid receptor)

BBa_K1633003
BBa_K1633003 Version 1 (Component)
MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

BBa_K1633006
BBa_K1633006 Version 1 (Component)
MOR siRNA-4 (siRNA for mouse Mu opioid receptor)

BBa_K658011
BBa_K658011 Version 1 (Component)
a bacteria population-control device with lux pR-3/5 driven by lacl+pL

BBa_K1124108
BBa_K1124108 Version 1 (Component)
pLambda-mCherry-pConst-c1 repressor generator (-LVA, -barcode, E234K) (for UV induction check)

BBa_K1444003
BBa_K1444003 Version 1 (Component)
Composite promoter and consensus <i>B. subtilis</i> RBS - <i>C1-&#955;</i> x3

BBa_K176092
BBa_K176092 Version 1 (Component)
Cell density controller: pCon max->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

BBa_K188006
BBa_K188006 Version 1 (Component)
Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be

BBa_J28028
BBa_J28028 Version 1 (Component)
lux autoinducer

BBa_K1497197
BBa_K1497197 Version 1 (Component)
B0034-CHI - Chalcone Isomerase from Petunia with strong RBS

BBa_K1036003
BBa_K1036003 Version 1 (Component)
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

BBa_K1541000
BBa_K1541000 Version 1 (Component)
promoter lux pR with riboregulator RR12y

BBa_K327015
BBa_K327015 Version 1 (Component)
Lux activated, C1lam repressed switch

BBa_K2050419
BBa_K2050419 Version 1 (Component)
RNA Polimerase III Promoter (H1 promoter)

BBa_J10064
BBa_J10064 Version 1 (Component)
Lux Operon Vibrio fischeri from part K325909

BBa_K2050422
BBa_K2050422 Version 1 (Component)
Mouse H1 promoter (RNA Polymerase III promoter)

BBa_K1541025
BBa_K1541025 Version 1 (Component)
sfGFP under promoter P(Lux) with riboregulator RR12y

BBa_J329001
BBa_J329001 Version 1 (Component)
Simple lux-based QS circuit expressing untagged GFP

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_K584011
BBa_K584011 Version 1 (Component)
Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP

BBa_I13211
BBa_I13211 Version 1 (Component)
Biobricked version of the natural Lux quorum sensing system

BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

BBa_K1014015
BBa_K1014015 Version 1 (Component)
Biological proportional operational Mu-circuit(1)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.