LigA
BBa_K525720 Version 1 (Component)
DNA ligase from Escherichia coli (LigA)

LigA
BBa_K525710 Version 1 (Component)
DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag

LigA
BBa_K525730 Version 1 (Component)
DNA ligase from Escherichia coli (LigA) with PT7 and RBS

LigA
BBa_K525740 Version 1 (Component)
DNA ligase from Escherichia coli (LigA) with His-tag

BBa_K606044
BBa_K606044 Version 1 (Component)
pVeg SpoVG cI repressor from phage 434 (+LVA) double terminator.

BBa_K606055
BBa_K606055 Version 1 (Component)
pVeg SpoVG cI repressor from phage 434 (+LVA)

BBa_K510023
BBa_K510023 Version 1 (Component)
Improved Flip Flop (Module II: asRNA)

BBa_K510036
BBa_K510036 Version 1 (Component)
Improved Flip Flop (Module II: asRNA)

Pbad-ChiXR
BBa_K564011 Version 1 (Component)
ara-->chiXR (sRNA) device

BBa_I13001
BBa_I13001 Version 1 (Component)
RBS B0030 w/ YFP (-LVA) TT

BBa_I13002
BBa_I13002 Version 1 (Component)
RBS B0031 w/ YFP (-LVA) TT

cI434 opt
BBa_K566011 Version 1 (Component)
cI434 repressor from phage 434 optimized for E. coli cI (+LVA)

cI opt
BBa_K566012 Version 1 (Component)
cI repressor from Lambda phage optimized for E. coli cI (+LVA)

RFP opt
BBa_K566013 Version 1 (Component)
RFP optimized for E. coli (+LVA)

BBa_K641021
BBa_K641021 Version 1 (Component)
P(cspA) - Stress Promoter

BBa_K641040
BBa_K641040 Version 1 (Component)
P(cysA) - Stress Promoter

BBa_K641043
BBa_K641043 Version 1 (Component)
P(gadA) - Stress Promoter

GFP-spore
BBa_K823049 Version 1 (Component)
P_<i>cotYZ</i> - <i>cotZ</i> (-2aa) - <i>gfp</i> - Terminator

BBa_K784027
BBa_K784027 Version 1 (Component)
XhoI+RBS+Alkaline Phosphatase (phoA) +XmaI

BBa_K759003
BBa_K759003 Version 1 (Component)
phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K759004
BBa_K759004 Version 1 (Component)
phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K759005
BBa_K759005 Version 1 (Component)
phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

BBa_K759006
BBa_K759006 Version 1 (Component)
B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_K759007
BBa_K759007 Version 1 (Component)
RBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K082003
BBa_K082003 Version 1 (Component)
GFP(+LVA)

BBa_K082024
BBa_K082024 Version 1 (Component)
B0034-GFP(LVA)-B0015

BBa_K082015
BBa_K082015 Version 1 (Component)
GFP(LVA)-B0015

BBa_K142016
BBa_K142016 Version 1 (Component)
tet-controlled lacI IS mutant (R197A) generator

BBa_K142018
BBa_K142018 Version 1 (Component)
tet-controlled lacI IS mutant (T276A) generator

BBa_K142020
BBa_K142020 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276A) generator

BBa_K142022
BBa_K142022 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276A) generator

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K142030
BBa_K142030 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette

SasA
BBa_K116502 Version 1 (Component)
SasA (Synechococcus adaptive sensor A)

BBa_C0361
BBa_C0361 Version 1 (Component)
luxI (+LVA) - Codon-optimised for E.coli

BBa_C0378
BBa_C0378 Version 1 (Component)
lasI (+LVA) - Codon-optimised for E.coli

BBa_M10044
BBa_M10044 Version 1 (Component)
{&#706;espP(beta)!} autotransporter

BBa_M10214
BBa_M10214 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;AG4&#707;}{&#706;espP(beta)!}{dblTerm}

BBa_M10222
BBa_M10222 Version 1 (Component)
{Pbad.rbs.prepro.StrepTag}{&#706;GS5-IILK&#707;}{&#706;espP(beta)!}{dblTerm}

BBa_K176001
BBa_K176001 Version 1 (Component)
PoPS->RBS+tetR(no LVA)+T

RIP
BBa_K237000 Version 1 (Component)
RIP - Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA)

sRIP
BBa_K237003 Version 1 (Component)
RIP +export signal (OmpA). Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA)

Plamda-GFP
BBa_I763011 Version 1 (Component)
promoter lambda (cI regulated) with GFP (+LVA) reporter

HA tag
BBa_T2009 Version 1 (Component)
hemagglutinin (HA) tag tail domain (YPYDVPDYA)

HA tag
BBa_T2010 Version 1 (Component)
hemagglutinin (HA) tag head domain (YPYDVPDYA)

HA tag
BBa_T2011 Version 1 (Component)
hemagglutinin (HA) tag special internal domain (YPYDVPDYA)

pirA
BBa_K273002 Version 1 (Component)
Pirin like protein (pirA) from <i>Synechocystis sp PCC6803</i>