BBa_K1962012
BBa_K1962012 Version 1 (Component)
A device for expression of Im-E3 in response to bile salts

BBa_K1962011
BBa_K1962011 Version 1 (Component)
A device for expression of Im-Ia in response to bile salts

RBS
BBa_K1642004 Version 1 (Component)
SD sequence of cpcB

BBa_K798005
BBa_K798005 Version 1 (Component)
Recognition sequence of ribozyme sequence

tuned pArs
BBa_K935002 Version 1 (Component)
E. coli ArsR under the control of pArs coupled with pArs + RFP

BBa_K176125
BBa_K176125 Version 1 (Component)
TetR repressible AHL->PoPS Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->PoPS

BBa_K861030
BBa_K861030 Version 1 (Component)
FadB, gene for fatty acid degradation from E.coli K12

BBa_K228851
BBa_K228851 Version 1 (Component)
Sal+supD+terminator

BBa_K1935016
BBa_K1935016 Version 1 (Component)
White Collar 2 under the control of pU6 promoter in C.elegans (pU6::WC2)

BBa_K1465209
BBa_K1465209 Version 1 (Component)
Carbonic anhydrase (csoS3), shell-proteins (csoS14) and pore protein (csoS1D) of the carboxysome

BBa_K1935014
BBa_K1935014 Version 1 (Component)
White Collar 1 under the control of pU6 promoter in C.elegans (pU6::WC1)

BBa_K2082232
BBa_K2082232 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)

BBa_K346004
BBa_K346004 Version 1 (Component)
RBS(B0034)_MBP(lead metal binding peptide egineered from PbrR)+Terminator(B0015)

BBa_K1315005
BBa_K1315005 Version 1 (Component)
The <i>clp</i> gene from <i>Xanthomonas campestris</i>

BBa_I746325
BBa_I746325 Version 1 (Component)
GFP under control of PLL promoter

BBa_K808028
BBa_K808028 Version 1 (Component)
PhoA: signal sequence for peri plasmic expression

BBa_K1315003
BBa_K1315003 Version 1 (Component)
The <i>rpfG</i> gene from <i>Xanthomonas campestris</i>

BBa_M31951
BBa_M31951 Version 1 (Component)
part of Gene II of M13K07

BBa_K1165012
BBa_K1165012 Version 1 (Component)
Ethylene Synthesis Cassette (SAM Synthetase, ACC Oxidase, ACC Synthase) under the Control of pTet Pr

BBa_I13412
BBa_I13412 Version 1 (Component)
CFP/YFP intermediate for screening plasmid v2

BBa_I759001
BBa_I759001 Version 1 (Component)
pTet-spacer-N

Ph-s
BBa_K143015 Version 1 (Component)
Promoter hyper-spank for B. subtilis

BBa_M302009
BBa_M302009 Version 1 (Component)
Region between HpaI site in gene II and BamHI site in gene III

BBa_K2074133
BBa_K2074133 Version 1 (Component)
pChlamy_3

BBa_K2122150
BBa_K2122150 Version 1 (Component)
Sequence for Gb3 Synthase

BBa_K090403
BBa_K090403 Version 1 (Component)
Gram-positive Shuttle Vector for Chromosomal Integration

Suffix-F
BBa_G1002 Version 1 (Component)
Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

BBa_J05218
BBa_J05218 Version 1 (Component)
Regulator for R4-cMaf

BBa_J05215
BBa_J05215 Version 1 (Component)
Regulator for R1-CREBH

GFP
BBa_M45413 Version 1 (Component)
Coding sequence for a green fluorescence protein back translated for homo sapiens

BBa_I6032
BBa_I6032 Version 1 (Component)
Promoter O_H Test R0040.E0430

BBa_K1315006
BBa_K1315006 Version 1 (Component)
The <i>manA</i> promoter from <i>Xanthomonas campestris</i>

BBa_K112247
BBa_K112247 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid

BBa_K112246
BBa_K112246 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid

BBa_K112248
BBa_K112248 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid

BBa_E0433
BBa_E0433 Version 1 (Component)
B0034.E0033.B0015

BBa_K2018032
BBa_K2018032 Version 1 (Component)
RBS for panK

BBa_K2018031
BBa_K2018031 Version 1 (Component)
Promoter for panKII

BBa_K759006
BBa_K759006 Version 1 (Component)
B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)

BBa_I741109
BBa_I741109 Version 1 (Component)
Lambda Or operator region

BBa_K176085
BBa_K176085 Version 1 (Component)
cell density->PoPS: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->PoPS

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K2018022
BBa_K2018022 Version 1 (Component)
The gene encoding phasin

BB base ve
BBa_I51020 Version 1 (Component)
BioBrick base vector (for constructing pSB**5 vectors)

BBa_K1491023
BBa_K1491023 Version 1 (Component)
C-terminus of T7 RNA Polymerase

BBa_K855003
BBa_K855003 Version 1 (Component)
coding sequence for pvdQ with histag added to the C-terminal followed by stop codon

BBa_K1361005
BBa_K1361005 Version 1 (Component)
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive