BBa_K518002
BBa_K518002 Version 1 (Component)
Firefly-renilla dual luciferase assay kit

BBa_K540000
BBa_K540000 Version 1 (Component)
<i>rcn-csgBAEFG</i>, over-induces adherence in response to cobalt

BBa_K540003
BBa_K540003 Version 1 (Component)
rcn-OmpR234, device that triggers adherence in response to cobalt.

BBa_K584020
BBa_K584020 Version 1 (Component)
AFP extracellular protein generator

BBa_K676005
BBa_K676005 Version 1 (Component)
Extractery

BBa_K607023
BBa_K607023 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 0.07RBS

BBa_K607024
BBa_K607024 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 0.3RBS

BBa_K607025
BBa_K607025 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 0.6RBS

BBa_K607026
BBa_K607026 Version 1 (Component)
PlasB_LasR-LVA_antipBad/araC with 1.0RBS

BBa_K607027
BBa_K607027 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 0.07RBS

BBa_K607028
BBa_K607028 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 0.3RBS

BBa_K607029
BBa_K607029 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 0.6RBS

BBa_K607030
BBa_K607030 Version 1 (Component)
PlasB_LasR-DAS_antipBad/araC with 1.0RBS

BBa_K607031
BBa_K607031 Version 1 (Component)
PlasB_LasR_antipBad/araC with 0.07RBS

BBa_K607032
BBa_K607032 Version 1 (Component)
PlasB_LasR_antipBad/araC with 0.3RBS

BBa_K607033
BBa_K607033 Version 1 (Component)
PlasB_LasR_antipBad/araC with 0.6RBS

BBa_K607034
BBa_K607034 Version 1 (Component)
PlasB_LasR_antipBad/araC with 1.0RBS

BBa_K649400
BBa_K649400 Version 1 (Component)
isoprene synthase (ispS)

BBa_K584026
BBa_K584026 Version 1 (Component)
Constitutive_Promoter + AFP(Antifreeze extra-cellular Protein Generator)

BBa_K613000
BBa_K613000 Version 1 (Component)
Medium-strength Plac promoter

BBa_K676014
BBa_K676014 Version 1 (Component)
Extractery

BBa_K676015
BBa_K676015 Version 1 (Component)
Extractery 2.0 - Deleted

BBa_K654034
BBa_K654034 Version 1 (Component)
Renilla Luciferase + Terminator

BBa_K654092
BBa_K654092 Version 1 (Component)
E. coli 1.0 RBS + Renilla Luciferase + Terminator

BBa_K654036
BBa_K654036 Version 1 (Component)
Anderson 1.0 Promoter Controlled Renilla Luciferase

BBa_K654037
BBa_K654037 Version 1 (Component)
Synechocystis psbA2 Promoter Controlled Renilla Luciferase

BBa_K654038
BBa_K654038 Version 1 (Component)
Synechocystis sigA promoter controlled Renilla Luciferase

BBa_K654093
BBa_K654093 Version 1 (Component)
Synechocystis psbA2 5'UTR Native RBS + Renilla Luciferase

BBa_K654094
BBa_K654094 Version 1 (Component)
Synechocystis psbA2 5'UTR Consensus RBS + Renilla Luciferase

BBa_K654045
BBa_K654045 Version 1 (Component)
Promoter Tester Anderson E.coli 1.0 Reference Dual Luciferase Assay

BBa_K654046
BBa_K654046 Version 1 (Component)
Promoter/RBS Tester Anderson 1.0 Reference Dual Luciferase Assay

BBa_K654047
BBa_K654047 Version 1 (Component)
Promoter Control Anderson E.coli 1.0 Reference Dual Luciferase Assay

BBa_K654048
BBa_K654048 Version 1 (Component)
Promoter Tester Native 6803 RBS psbA2 Reference Dual Luciferase Assay

BBa_K654049
BBa_K654049 Version 1 (Component)
Promoter/RBS Tester psbA2 Reference Dual Luciferase Assay

BBa_K654050
BBa_K654050 Version 1 (Component)
Promoter Control psbA2 Reference Dual Luciferase Assay

BBa_K654054
BBa_K654054 Version 1 (Component)
Promoter Tester 6803 Consensus RBS psbA2 Reference Dual Luciferase Assay

BBa_K654051
BBa_K654051 Version 1 (Component)
Promoter Tester 6803 Native RBS sigA Reference Dual Luciferase Assay

BBa_K654052
BBa_K654052 Version 1 (Component)
Promoter/RBS Tester sigA Reference Dual Luciferase Assay

BBa_K654053
BBa_K654053 Version 1 (Component)
Promoter Control sigA Reference Dual Luciferase Assay

BBa_K654055
BBa_K654055 Version 1 (Component)
Promoter Tester 6803 Consensus RBS sigA Reference Dual Luciferase Assay

BBa_K654096
BBa_K654096 Version 1 (Component)
E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)

BBa_K654097
BBa_K654097 Version 1 (Component)
Anderson 0.10 E. coli Reference Promoter

BBa_K654098
BBa_K654098 Version 1 (Component)
Anderson 0.01 E. coli Reference Promoter

BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.