BBa_K2018000
BBa_K2018000 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K2018036
BBa_K2018036 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K2018033
BBa_K2018033 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

enhanced
BBa_K2075011 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

enhanced G
BBa_K2075012 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K1123017
BBa_K1123017 Version 1 (Component)
DNA Coding Sequence for EGFP Protein

BBa_K1014016
BBa_K1014016 Version 1 (Component)
HrpS Protein Generator

BBa_I721004
BBa_I721004 Version 1 (Component)
Lead Binding Protein + Promoter (version a)

BBa_J26010
BBa_J26010 Version 1 (Component)
LuxR + AHL used to turn on GFP

BBa_K125550
BBa_K125550 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J45010
BBa_J45010 Version 1 (Component)
JAMT: Jasmonic acid carboxyl methyltransferase: Produces jasmine scent

BBa_J133568
BBa_J133568 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133564
BBa_J133564 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133573
BBa_J133573 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133563
BBa_J133563 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133572
BBa_J133572 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133579
BBa_J133579 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133566
BBa_J133566 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133570
BBa_J133570 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133569
BBa_J133569 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133578
BBa_J133578 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133580
BBa_J133580 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133565
BBa_J133565 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133562
BBa_J133562 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133581
BBa_J133581 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133567
BBa_J133567 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133571
BBa_J133571 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K1777012
BBa_K1777012 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777014
BBa_K1777014 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1777013
BBa_K1777013 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1434002
BBa_K1434002 Version 1 (Component)
cAMP receptor protein (CRP) mutant

BBa_K648006
BBa_K648006 Version 1 (Component)
Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix

BBa_K1947028
BBa_K1947028 Version 1 (Component)
Improve the protein purification.

BBa_K1947027
BBa_K1947027 Version 1 (Component)
Improve the protein purification.

BBa_K1947026
BBa_K1947026 Version 1 (Component)
Improve the protein purification.

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K1014019
BBa_K1014019 Version 1 (Component)
HrpR Protein Generator(M)

BBa_K1091003
BBa_K1091003 Version 1 (Component)
green fluorescent protein－wt

BBa_K1777004
BBa_K1777004 Version 1 (Component)
sequence-specific RNA binding protein

BBa_K1091002
BBa_K1091002 Version 1 (Component)
green fluorescent protein－slow