BBa_K2036024
BBa_K2036024 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase

BBa_M31116
BBa_M31116 Version 1 (Component)
M13 Gene VIII terminator and promoter of Gene X

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor

cln2
BBa_K105014 Version 1 (Component)
cln2 PEST destabilization domain for rapid protein turnover

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

BBa_K648123
BBa_K648123 Version 1 (Component)
RecA with mutation of Lys 286 and Arg 243

BBa_J37021
BBa_J37021 Version 1 (Component)
pTetR Transfer Function with aTc

BBa_J107021
BBa_J107021 Version 1 (Component)
aTc sensor (J23106 promoter) with GFP

BBa_K1045009
BBa_K1045009 Version 1 (Component)
Terminator BBa_B0015 with inversed Pre- and Suffix

BBa_K1172914
BBa_K1172914 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO GFP)

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_I758601
BBa_I758601 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K1351019
BBa_K1351019 Version 1 (Component)
Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

BBa_K321002
BBa_K321002 Version 1 (Component)
eGFP BC part

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

kanA
BBa_K349115 Version 1 (Component)
kanR-AB (BioBytes 2.0)

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

GFP-AID
BBa_K812110 Version 1 (Component)
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

BBa_K137021
BBa_K137021 Version 1 (Component)
GFP with (AC)20 repeat after start codon

BBa_K2150010
BBa_K2150010 Version 1 (Component)
This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon