BBa_K337060
BBa_K337060 Version 1 (Component)
Fragment 3 of wt AAV1

BBa_K1413042
BBa_K1413042 Version 1 (Component)
OriVR6Kgamma origin of replication (ori) 2

7x His
BBa_T2001 Version 1 (Component)
7x His tag head domain

BBa_K564023
BBa_K564023 Version 1 (Component)
atC-->chiX

BBa_J70622
BBa_J70622 Version 1 (Component)
RFC12 cMyc Tag Head Domain

BBa_J70623
BBa_J70623 Version 1 (Component)
RFC12 cMyc Tag Tail Domain

BBa_J70624
BBa_J70624 Version 1 (Component)
RFC12 FLAG Tag Head Domain

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_M31786
BBa_M31786 Version 1 (Component)
1st half of Gene III- preBamHI cut

BBa_K648103
BBa_K648103 Version 1 (Component)
RecA with mutation of Arg 243

CsgD
BBa_K1019001 Version 1 (Component)
CsgD: positive regulator of curlin genes

BBa_K648102
BBa_K648102 Version 1 (Component)
RecA with mutation of Lys 286

BBa_K137035
BBa_K137035 Version 1 (Component)
Device with GFP with (AC)22 repeat after start codon

BBa_K137034
BBa_K137034 Version 1 (Component)
Device with GFP with (AC)20 repeat after start codon

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K325905
BBa_K325905 Version 1 (Component)
Bacterial Lux reporter <BR> CDEG p&#955; AB<BR><i>(E. coli optimised)</i>

BBa_K2123202
BBa_K2123202 Version 1 (Component)
MerT + MerP (Hg transporters) + MerB (Organomercurial Lyase) + MerA (Mercuric Reductase) + B0015

BBa_J70621
BBa_J70621 Version 1 (Component)
RFC12 7x His Tag Tail Domain

BBa_I733005
BBa_I733005 Version 1 (Component)
Produce GFP in presence of AHL

BBa_K1725317
BBa_K1725317 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K590044
BBa_K590044 Version 1 (Component)
ADC-PSB1A3-High constitutive

BBa_K590036
BBa_K590036 Version 1 (Component)
ADC-PSB3K3-High consititutive

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_J11009
BBa_J11009 Version 1 (Component)
variant of J11005 (low temp color) with stronger RBS

BBa_K1947017
BBa_K1947017 Version 1 (Component)
We verify the expression effect of Mms13.

BBa_J14462
BBa_J14462 Version 1 (Component)
Composite part comprised of J13002 and J04650

BBa_K351000
BBa_K351000 Version 1 (Component)
Signal peptide for cell surface of fission yeast

BBa_K1051262
BBa_K1051262 Version 1 (Component)
The measurement pathway of degradation tag K1051208.

BBa_K1051260
BBa_K1051260 Version 1 (Component)
The measurement pathway of degradation tag K1051206.

BBa_K1051261
BBa_K1051261 Version 1 (Component)
The measurement pathway of degradation tag K1051207.

BBa_J14459
BBa_J14459 Version 1 (Component)
Composite part comprised of J04500 and J04630

BBa_J14464
BBa_J14464 Version 1 (Component)
Composite part comprised of J13002 and J04630

BBa_K1676088
BBa_K1676088 Version 1 (Component)
Mutant 12 of LDH and wildtype LDP

BBa_K783048
BBa_K783048 Version 1 (Component)
This is a MoClo converted version of BBa_B0031

BBa_K783039
BBa_K783039 Version 1 (Component)
This is a MoClo converted version of BBa_J23101

BBa_K783049
BBa_K783049 Version 1 (Component)
This is a MoClo converted version of BBa_B0032

BBa_K783040
BBa_K783040 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K1413045
BBa_K1413045 Version 1 (Component)
A fusion of Universal Transposon Plasmid and pSB1C3

BBa_K783034
BBa_K783034 Version 1 (Component)
This is a MoClo converted version of BBa_J23114

BBa_K783050
BBa_K783050 Version 1 (Component)
This is a MoClo converted version of BBa_B0033

BBa_K783038
BBa_K783038 Version 1 (Component)
This is a MoClo converted version of BBa_J23100

BBa_K2036028
BBa_K2036028 Version 1 (Component)
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase

BBa_K1676091
BBa_K1676091 Version 1 (Component)
Mutant 15 of LDH and wildtype LDP

BBa_K1676086
BBa_K1676086 Version 1 (Component)
Mutant 10 of LDH and wildtype LDP

BBa_K1676080
BBa_K1676080 Version 1 (Component)
Mutant 4 of LDH and wildtype LDP

BBa_K2036027
BBa_K2036027 Version 1 (Component)
pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag

BBa_I745014
BBa_I745014 Version 1 (Component)
pLV-UBC-siGata3-3'-GFP

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)