TD0.3
BBa_K750011 Version 1 (Component)
TIME DELAY0.3:LuxI(RBS0.3)->LuxR->LuxPR->GFP

TD0.01
BBa_K750012 Version 1 (Component)
TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_J24816
BBa_J24816 Version 1 (Component)
J23101 promoter with Luc gene attached

rTT+pR
BBa_K093003 Version 1 (Component)
Convergent Promoter System: Forward Module: rTT+lambda pR

BBa_K124017
BBa_K124017 Version 1 (Component)
Bacteriophage Lysis Cassette S105, R, and Rz

BBa_K106342
BBa_K106342 Version 1 (Component)
AB shuttle vector

BBa_K106343
BBa_K106343 Version 1 (Component)
BD shuttle vector

pBSEP1
BBa_K090401 Version 1 (Component)
Gram-positive Shuttle Vector for Episomal Expression

rPlac+TT
BBa_K093004 Version 1 (Component)
Convergent Promoter System: Reverse Module: rPlac+TT

BBa_K156027
BBa_K156027 Version 1 (Component)
Genetic attenuator (synthetic transcription terminator) ~68% efficient

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

BBa_K142024
BBa_K142024 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette

BBa_K156028
BBa_K156028 Version 1 (Component)
Genetic attenuator (synthetic transciption terminator) ~45% efficient

BBa_K156029
BBa_K156029 Version 1 (Component)
Genetic attenuator (synthetic transcription terminator) ~17% efficient

BBa_K142025
BBa_K142025 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette

BBa_K142026
BBa_K142026 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276A) and TetR expression cassette

BBa_K156030
BBa_K156030 Version 1 (Component)
Genetic attenuator measurement device (template)

BBa_K142027
BBa_K142027 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (T276F) and TetR expression cassette

BBa_K142028
BBa_K142028 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276A)/TetR expression cassette

BBa_K142029
BBa_K142029 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197A, T276F)/TetR expression cassette

BBa_K142030
BBa_K142030 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276A)/TetR expression cassette

BBa_K142031
BBa_K142031 Version 1 (Component)
IPTG-on tetracycline-off pulse generator with LacI mutant (R197F, T276F)/TetR expression cassette

BBa_K142073
BBa_K142073 Version 1 (Component)
lac-controlled GFP generator with constitutive lacI expression cassette

BBa_K142074
BBa_K142074 Version 1 (Component)
lac-controlled GFP-LVA generator with constitutive lacI expression cassette

BBa_K142075
BBa_K142075 Version 1 (Component)
lac-controlled CFP generator with constitutive lacI expression cassette

BBa_K142076
BBa_K142076 Version 1 (Component)
lac-controlled RFP generator with constitutive lacI expression cassette

BBa_K142048
BBa_K142048 Version 1 (Component)
promotorless lacI expression cassette followed by constitutively active TetR generator

BBa_K165061
BBa_K165061 Version 1 (Component)
pRS304* yeast shuttle vector, TRP1 selection

BBa_K165060
BBa_K165060 Version 1 (Component)
pRS303 yeast shuttle vector, HIS3

BBa_K165062
BBa_K165062 Version 1 (Component)
pRS305 yeast shuttle vector, LEU2 selection

BBa_K165063
BBa_K165063 Version 1 (Component)
pRS306 yeast shuttle vector, URA3 selection

BBa_K090403
BBa_K090403 Version 1 (Component)
Gram-positive Shuttle Vector for Chromosomal Integration

BBa_K122021
BBa_K122021 Version 1 (Component)
Cassette3: Constitutive TAL with integration site

trfA
BBa_K112131 Version 1 (Component)
TrfA constitutive expression cassette

BBa_K112133
BBa_K112133 Version 1 (Component)
Xis Int temperature sensitive expression cassette

BBa_K112140
BBa_K112140 Version 1 (Component)
attR1 rev-CamR-Lox-AmpR

BBa_K112142
BBa_K112142 Version 1 (Component)
attR2 recombination site-reverse orientation

BBa_K112141
BBa_K112141 Version 1 (Component)
attR2 recombination site

BBa_K112135
BBa_K112135 Version 1 (Component)
Phagemid-attR1-R6K-attR2

BBa_K112136
BBa_K112136 Version 1 (Component)
Phagemid-attR1-R6K-attR2-CmR

BBa_K112137
BBa_K112137 Version 1 (Component)
Xis Int & TrfA combined expression cassette

BBa_K112138
BBa_K112138 Version 1 (Component)
FRT-KanR-R6K-FRT-attP21

BBa_J70028
BBa_J70028 Version 1 (Component)
tetM tetracycline resistance cassette

BBa_K116634
BBa_K116634 Version 1 (Component)
RBS + LuxR + RBS + LuxI + T

BBa_K116635
BBa_K116635 Version 1 (Component)
pRE + RBS + LuxI + T

BBa_K116638
BBa_K116638 Version 1 (Component)
pLac + RBS + LuxI + T

BBa_K091220
BBa_K091220 Version 1 (Component)
RBS+TetA(C)+TT

BBa_M11059
BBa_M11059 Version 1 (Component)
pfaB gene for omega-3 fatty acid synthesis